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Structural systems biology evaluation of metabolic thermotolerance in Escherichia coli

ABSTRACT: Investigation of whole genome gene expression level in E. coli K-12 MG1655 in glucose M9 minimal media with/without heatshock

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

PROVIDER: GSE42675 | GEO | 2013/06/20

SECONDARY ACCESSION(S): PRJNA182717

REPOSITORIES: GEO

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Structural systems biology evaluation of metabolic thermotolerance in Escherichia coli

Project description:Investigation of whole genome gene expression level in E. coli K-12 MG1655 in glucose M9 minimal media with/without heatshock A six chip study using total RNA recovered from E. coli K-12 MG1655 grown up to OD600nm 0.6 (mid-exponential phase) in M9 minimal media supplemented with 0.2% glucose with/without heatshock in 42oC. The high-density oligonucleotide tiling arrays used were consisted of 371,034 oligonucleotide probes with 50-bp length that are spaced 25 bp apart across the E. coli genome (NimbleGen). Experiments were performed with three biological replicates.

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