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TGF-beta 1 effect on primary bovine aortic endothelial cells

ABSTRACT: Analysis of primary bovine aortic endothelial cells treated for 24 hours with TGF-beta 1 5 ng/ml. TGF-beta 1 has been shown to induce endothelial-to-mesenchymal transition (EndoMT) and to be implicated in differentiation of endothelial cells into smooth muscle-like cells as occurred in vascular neointimal formation.

ORGANISM(S): Bos taurus

PROVIDER: GSE42932 | GEO | 2013/04/10

SECONDARY ACCESSION(S): PRJNA183888

REPOSITORIES: GEO

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TGF-beta 1 effect on primary bovine aortic endothelial cells

Project description:Analysis of primary bovine aortic endothelial cells treated for 24 hours with TGF-beta 1 5 ng/ml. TGF-beta 1 has been shown to induce endothelial-to-mesenchymal transition (EndoMT) and to be implicated in differentiation of endothelial cells into smooth muscle-like cells as occurred in vascular neointimal formation. Primary aortic endothelial cells seeded on 10 mm diameter plates were incubated with TGF-beta 1 (5 ng/ml) for 24 hours or left under basal conditions. Triplicates from three different cultures.

2013-04-10 | E-GEOD-42932 | biostudies-arrayexpress

TGF-β regulated RNA-binding proteins control endothelial function and mesenchymal transition in cardiac endothelial cells [RNA-seq]

Project description:Endothelial to mesenchymal transition (EndoMT) plays a key role in heart development, but is also implicated in cardiovascular diseases in postnatal life. While the roles of TGF-β as inducer of EndoMT on the transcriptional level are well characterised, its post-transcriptional regulatory mechanisms remain largely unknown. Here, we identified global changes in the endothelial mRNA bound proteome upon TGF-β stimulation using RNA interactome capture. Characterisation of TGF-β regulated RNA binding proteins (RBPs) revealed heterogeneous nuclear ribonucleoprotein H1 (hnRNP H1) and Cold Shock Domain Containing E1 (Csde1) as key regulators of endothelial function and EndoMT. We profiled TGF-β driven changes in the RNA binding patterns of hnRNP H1 and Csde1 and found that they dynamically bind and regulate specific subsets of functionally connected RNAs related to mesenchymal activation upon TGF-β stimulation. Together, we show that RBPs play a key role in EndoMT and that the RBPs hnRNP H1 and Csde1 maintain endothelial cell function and counteract mesenchymal activation.

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Bovine Glomerular and Aortic Endothelial Cells

Project description:SAGE libraries were prepared from mRNA of cultured bovine glomerular and aortic endothelial cells. Cells both cell lines were positive for acetylated LDL uptake and vWF expression, and were proven to be free of non-endothelial cells. Cultures were studied at passage 9-10. The cells were confluent, cultured in parallel, under identical conditions. Cells were plated on gelatin coated cell culture plastic in RPMI 1640 medium containing 10% FBS and streptomycin/penicillin. The last medium change was 48 hours prior to mRNA harvest. Keywords = endothelial, glomerular, kidney, aorta, Keywords: other

2002-12-09 | E-GEOD-110 | biostudies-arrayexpress

Bovine Glomerular and Aortic Endothelial Cells

2002-12-10 | GSE110 | GEO

SILAC Bovine Aortic Endothelial Cells

Project description:This is a pilot SILAC study wherein bovine aortic endothelial cells in culture were treated with the oxidant peroxynitrite, and compared to a control condition that consisted of reverse addition generated from peroxynitrite that underwent complete decomposition prior to mixing with cells. The study targets the protein cytochrome c, and hence, the results were confined to a search of all proteins under 45 kDa. The goal of this pilot study were to: 1) Identify the presence of cytochrome c in nuclear and cytosolic fractions 2) Identify amino acid residues in cytochrome c presenting post-translational modifications, such as tyrosine nitration or methionine oxidation

2023-08-06 | PXD035830 | JPOST Repository

Bos taurus

Project description:TGF-beta 1 effect on primary bovine aortic endothelial cells

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Genes regulated by TGF-beta in bovine articular chondrocytes

Project description:Bovine articular chondrocytes were grown in micromass culture and were either untreated or treated with 5 ng TGF-b1/ml for 8 hours to identify genes regulated by TGF-b. Six total samples were analyzed. Three biological replicates of untreated bovine articular chondrocytes grown in micromass culture and three biological replicates of bovine articular chondrocytes grown in micromass culture and treated with 5ng TGF-b1/ ml for 8 hours.

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Genes regulated by TGF-beta in bovine articular chondrocytes

Project description:Bovine articular chondrocytes were grown in micromass culture and were either untreated or treated with 5 ng TGF-b1/ml for 8 hours to identify genes regulated by TGF-b.

2011-10-01 | GSE29233 | GEO

Effect of PDGF, TGF-β and miR-221 on gene expression during proliferation and differentiation of human aortic smooth muscle cells

Project description:To investigate the key molecular and mechanism during the proliferation and differentiation of human aortic smooth muscle cells, we treated HASMC cell lines with PDGF, TGF-β and miR-221.We then performed gene expression profiling analysis using data obtained from ONT long-read RNA-seq of 4 different treatment.

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