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Quantitative proteomics cell bilayer (qPCB) method for studying cell-cell communication

ABSTRACT: Cellular communication is a fundamental process in biology. We developed an in vitro procedure for quantitatively analyzing proteome-wide changes triggered by interaction of different cell types. Using an adipocyte-macrophage bilayer co-culture model we detected thousands of proteins and deciphered regulatory pathways involved in low-grade inflammation leading to insulin resistance. The method can be applied for multiple cell-cell combinations to gain understanding in cellular interaction.

ORGANISM(S): Mus musculus

PROVIDER: GSE43426 | GEO | 2015/01/01

SECONDARY ACCESSION(S): PRJNA186076

REPOSITORIES: GEO

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