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The expression profiles of WT and sgs1△ after tangeretin treatment

ABSTRACT: Tangeretin is a member of the polymethoxyflavones in citrus peel, which have been of interest due to their anti-atherogenic, anti-inflammatory, and anti-carcinogenic properties. However, their detailed target pathways have not been fully discovered. We applied the notion of chemical-genetic and genetic interactions in budding yeast, screening the putative targeting pathways of tangeretin, and we found that sgs1△ yeast was sensitive to tangeretin treatment. Therefore, we applied this microarray test to investigate the potential pathways influenced by tangeretin in sgs1. The gene expression profiles of WT and sgs1△ mutated yeast under tangeretin treatment were compared.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE43799 | GEO | 2013/01/29

SECONDARY ACCESSION(S): PRJNA187512

REPOSITORIES: GEO

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The expression profiles of WT and sgs1△ after tangeretin treatment

Project description:Tangeretin is a member of the polymethoxyflavones in citrus peel, which have been of interest due to their anti-atherogenic, anti-inflammatory, and anti-carcinogenic properties. However, their detailed target pathways have not been fully discovered. We applied the notion of chemical-genetic and genetic interactions in budding yeast, screening the putative targeting pathways of tangeretin, and we found that sgs1△ yeast was sensitive to tangeretin treatment. Therefore, we applied this microarray test to investigate the potential pathways influenced by tangeretin in sgs1. The gene expression profiles of WT and sgs1△ mutated yeast under tangeretin treatment were compared. The gene expression profiles of S. cerevisiae WT and sgs1△ strains under 30 μM tangeretin treatment for 4 hours were compared. Triplicated tests were made for each sample (tangeretin-treated WT, control-treated WT, tangeretin-treated sgs1△, control-treated sgs1△).

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