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MiR-146a-5p expression effect on human mesenchymal stem cell

ABSTRACT: Analysis of human mesenchymal stem cells (MSC) from bone marrow and the Wharton's jelly of the umbilical cord after manupulating miR-146a-5p expression. miR-146a-5p is involved in controlling the proliferation and migration of MSCs. Results provide miR-146a-5p-regulating genes in MSCs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE44186 | GEO | 2013/09/05

SECONDARY ACCESSION(S): PRJNA189045

REPOSITORIES: GEO

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miR-146a-5p expression effect on human mesenchymal stem cell

Project description:Analysis of human mesenchymal stem cells (MSC) from bone marrow and the Wharton's jelly of the umbilical cord after manupulating miR-146a-5p expression. miR-146a-5p is involved in controlling the proliferation and migration of MSCs. Results provide miR-146a-5p-regulating genes in MSCs. In this study, BM-MSCs transduced with miR-146a-5p expression vector or pCDH-CMV-MCS-EF1-copGFP vector only, as well as two WJ-MSCs transfected with short interfering RNAs targeting miR-146a or a GFP control.

2013-09-05 | E-GEOD-44186 | biostudies-arrayexpress

miR-146a-5p circuitry uncouples cell proliferation and migration, but not differentiation, in human mesenchymal stem cells

Project description:Administration of mesenchymal stem cells (MSCs) has the potential to ameliorate degenerative disorders and to repair damaged tissues. The homing of transplanted MSCs to injured sites is a critical property of engraftment. Our aim was to identify microRNAs involved in controlling MSC proliferation and migration. MSCs can be isolated from bone marrow and umbilical cord Wharton's jelly (BM-MSCs and WJ-MSCs, respectively), and WJ-MSCs show poorer motility yet have a better amplification rate compared to BM-MSCs. One human BM-MSC (pooled sample of 4 independent donors), one human WJ-MSC (pooled sample of 3 independent donors), and differentiated osteocytes and adipocytes derived from BM-MSCs after 2 weeks induction.

2013-09-05 | E-GEOD-46989 | biostudies-arrayexpress

miR-146a-5p circuitry uncouples cell proliferation and migration, but not differentiation, in human mesenchymal stem cells

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Extracellular Vesicles Derived from Wharton’s Jelly Mesenchymal Stem Cells Inhibit Inflammatory Responses to SARS-CoV-2 and Influenza Viral Proteins via the miR-146a/ NF-κB pathway

Project description:Purpose: small RNAseq analyses were conducted to identify the key miRNAs which involves in the suppression of inflammatory respons via NF-kB pathway in the Wharton's Jelly Mesehchymal Stem Cell-derived EV (WJ-EV). Methode: WJ-MSC were cultured using EV-Depleted medium for 24 hr. for hWJ-EV, after reach 80% confluences the cells were cultured under a hypoxic condition (1%O2) for 48 hr. The conditioned medium from WJ-MSC and hWJ-MSC then were collected and centrifuged to get the EVs. Result: miR-21, miR-143, and miR-146a were involved in the regulation of the NF-κB signaling pathway and inflammatory cytokines.

2024-12-01 | GSE273241 | GEO

miR-146a-5p contributes to inflammation-mediated β cell mitochondrial dysfunction and apoptosis

Project description:MicroRNAs (miRNAs) are small non-coding RNAs that function as modulators of gene expression. We previously showed that miR-146a-5p is upregulated in pancreatic islets treated with pro-inflammatory cytokines and in pancreatic sections from organ donors with type 1 diabetes (T1D). Other studies have associated overexpression of miR-146a-5p with β cell apoptosis and impaired insulin secretion; however, the molecular mechanisms mediating these effects remain elusive. To investigate the role of miR-146a-5p in β cell function, we developed stable MIN6 cell lines transduced with lentiviral vectors to either overexpress or inhibit the expression of miR-146a-5p. Monoclonal cell populations were treated with pro-inflammatory cytokines (IL1β, IFNg, and TNFα) to model T1D in vitro. We found that overexpression of miR-146a-5p increased the cell death of MIN6 cells under inflammatory stress, whereas inhibition of miR-146a-5p reversed these effects. Additionally, inhibition of miR-146a-5p increased mitochondrial DNA copy number, respiration rate, and ATP production, suggesting that miR-146a-5p inhibition improves mitochondrial function. In support of this finding, we also observed that miR-146a-5p is enriched in the mitochondria of MIN6 cells treated with cytokines. Consistently, bioinformatic analysis of RNA sequencing data using MIN6 stable cells showed enrichment of pathways related to insulin secretion, apoptosis, and mitochondrial function when the expression levels of miR-146a-5p were altered. Overall, the findings from our study show for the first time that miR-146a-5p upregulation during inflammatory stress may promote β cell dysfunction and death by suppressing mitochondrial function.

2024-04-10 | GSE255756 | GEO

RNA-seq of miR-146a-5p overexpressing T24 human bladder cancer cells

Project description:We established stable miR-146a-5p overexpression T24 cells, then performed transcriptome profiling of miR-146a-5p overexpressing cells compared to control T24 cells to detect the molecular mechanisms of the miR-146a-5p’s effect on bladder cancer cells.

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Molecular pathways reflecting poor intrauterine growth are imprinted in WhartonM-bM-^@M-^Ys jelly derived Mesenchymal Stem Cells [Set2]

Project description:In order to identify gene-expression patterns in mesenchymal stem cells associated with different birth weights and intrauterine growth parameters, we conducted comparative microarray gene expression experiments prior and post acute insulin stimulation Mesenchymal stem cells (MSC) were extracted from the Wharton's jelly of the umbilical cord of normal and SGA babies. The MSCs were expended in culture. The MSCs were subjected to acute insulin treatment for various time points. The RNA of these cells were collected for gene-expression study.

2014-03-31 | E-GEOD-52842 | biostudies-arrayexpress

Molecular pathways reflecting poor intrauterine growth are imprinted in WhartonM-bM-^@M-^Ys jelly derived Mesenchymal Stem Cells [set4]

2014-03-31 | E-GEOD-52844 | biostudies-arrayexpress

Molecular pathways reflecting poor intrauterine growth are imprinted in WhartonM-bM-^@M-^Ys jelly derived Mesenchymal Stem Cells [set3]

2014-03-31 | E-GEOD-52843 | biostudies-arrayexpress

Whole transcript expression profiling of umbilical cord- and bone marrow-derived stem cells

Project description:Mesenchymal stromal cells (MSCs) are multipotent stem cells with potent immunosuppressive and trophic support functions. Although bone marrow is considered the golden standard to isolate classical MSCs (BM-MSC), MSC-like cells are currently also derived from other, more easily accessible extra-embryonic tissues such as the umbilical cord. In this study we compared the gene expression profile of human Wharton's jelly explant-derived MSC cultures with two adult MSC populations derived from bone marrow, namely BM-MSC and multipotent adult progenitor cells (MAPC). Here we demonstrate, by using genome wide gene expression analysis, that WJ-MSCs intrinsically have a differential gene expression profile compared to the adult MSCs. Gene ontology analysis revealed an increased expression of genes associated with cell-adhesion, proliferation, and immune system functioning. Furthermore, in comparison to adult MSC, stem cells from the Wharton’s jelly highly express genes involved in neurotrophic support (e.g. LIF, BDNF, NTF3). Such enhanced signatures make WJ-MSC an attractive candidate for cell-based therapy in neurodegenerative and immune-mediated CNS disorders such as multiple sclerosis or amyotrophic lateral sclerosis.

2017-12-28 | GSE77685 | GEO

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