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DNA-damaging agent and immune inducer synergistically induced defence gene expression in Arabidopsis

ABSTRACT: We used microarrays to identify differentially expressed genes after DNA-damaging agent bleomycin (BLM) and/or immune inducer 2, 6-dichloroisonicotinic acid (INA) treatment. We focused on those genes that were synergistically induced by co-treatment (BLM+INA).

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE44793 | GEO | 2013/11/14

SECONDARY ACCESSION(S): PRJNA191812

REPOSITORIES: GEO

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DNA-damaging agent and immune inducer synergistically induced defence gene expression in Arabidopsis

Project description:We used microarrays to identify differentially expressed genes after DNA-damaging agent bleomycin (BLM) and/or immune inducer 2, 6-dichloroisonicotinic acid (INA) treatment. We focused on those genes that were synergistically induced by co-treatment (BLM+INA). Arabidopsis seedlings were treated with 4 μg/ml BLM and/or low INA (10 μM). There are 4 treatments: control (CK), INA, BLM and BLM+INA. Each treatments have three biological replicates. There are 12 samples in total.

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E-GEOD-44793

Project description:Transcription profiling by array of Arabidopsis seedlings grown after DNA-damaging agent bleomycin (BLM) and/or immune inducer 2, 6-dichloroisonicotinic acid (INA) treatment to identify synergistically induced defence genes

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Project description:Onconase represents a new class of RNA-damaging drugs. Mechanistically, Onconase is thought to internalize, where it degrades intracellular RNAs such as tRNA and double-stranded RNA, and thereby suppresses protein synthesis. However, there may be additional or alternative mechanism(s) of action. Microarray analysis was used to compare gene expression profiles in untreated human malignant mesothelioma (MM) cell lines and cells exposed to 5 ug/ml Onconase for 24 h.

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