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RAW264.7 cells: RANKL vs Bone Metastasis Tumor Cell Condidtioned Media

ABSTRACT: Transcriptional profiling of differential miRNA expression in mouse RAW264.7 preosteoclast cells comparing control untreated cells with RAW264.7 cells treated for 6 days. Treatment conditiones tested included 50ng/mL RANKL or indicated bone metastasis tumor cell conditioned media from 4T1, 4T1.2, TSU-PR1, and TSU-PR1-B2 cell lines.

ORGANISM(S): Rattus norvegicus Mus musculus Danio rerio Homo sapiens Caenorhabditis elegans Drosophila melanogaster

PROVIDER: GSE44936 | GEO | 2013/10/14

SECONDARY ACCESSION(S): PRJNA192552

REPOSITORIES: GEO

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RAW264.7 cells: RANKL vs Bone Metastasis Tumor Cell Condidtioned Media

Project description:Transcriptional profiling of differential miRNA expression in mouse RAW264.7 preosteoclast cells comparing control untreated cells with RAW264.7 cells treated for 6 days. Treatment conditiones tested included 50ng/mL RANKL or indicated bone metastasis tumor cell conditioned media from 4T1, 4T1.2, TSU-PR1, and TSU-PR1-B2 cell lines. Two-condition experiment, Control cells vs. treated cells.

2013-10-14 | E-GEOD-44936 | biostudies-arrayexpress

RNA-seq in Cpeb4 knockdown RAW264.7 cells three days after RANKL stimulation

Project description:To identify the changes of mRNA expressions by Cpeb4 knockdown in RAW264.7 cells treted with RANKL for three days, we performed RNA-sequencing.

2024-08-31 | GSE241811 | GEO

Proteomic Study of Different Culture Medium Serum Volume Fractions on RANKL-dependent Differentiating into Osteoclasts of RAW264.7 Cells

Project description:Supplemental data for the article: Proteomic Study of Different Culture Medium Serum Volume Fractions on RANKL-dependent Differentiating into Osteoclasts of RAW264.7 Cells

2015-06-02 | PXD001935 | Pride

Expression data from 48 h incubation of RAW264.7 cells with 50 ng/ml RANK Ligand

Project description:A variety of genes are responsible for regulating osteoclastogenesis in response to RANK Ligand. Microarray analysis was used to identify genes sensitive to RANKL-induced osteoclastogenesis in RAW264.7 cells. RAW264.7 cells were incubated with 50 ng/mL RANKL or vehicle control (3 replicates each). After 48 h, total RNA were harvested by an RNeasy Plus Mini Kit (QIAGEN).

2015-11-11 | E-GEOD-74847 | biostudies-arrayexpress

lncRNA and mRNA expression profiles during osteoclastogenesis

Project description:Total RNA were extracted from osteoclasts differentiated from RAW264.7 cells at different stages: Monocytes (0h), Pre-osteoclasts (24h), Mature osteoclasts (72h) and activated osteoclasts (96h). LncRNA and mRNA expression profiles were decided by microarray. RAW264.7 cells were cultured with RANKL (100ng/ml) and M-CSF (50ng/ml) for 0h, 24h, 72h and 96h. TRAP stain, FAK stain, pit formation assay, fusion assay and q-PCR were performed to validate the in vitro model.

2015-08-28 | E-GEOD-72478 | biostudies-arrayexpress

RNA-seq in Cpeb4 knockdown RAW264.7 cells three days after RANKL stimulation

Project description:RNA-seq in Cpeb4 knockdown RAW264.7 cells three days after RANKL stimulation

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Expression data from 48 h incubation of RAW264.7 cells with 50 ng/ml RANK Ligand

2015-11-11 | GSE74847 | GEO

Effect of Cyclin-dependent kinase 2 (CINP) inhibition on TSU-PR1 cell line with stable KLF5 expression May

Project description:To study wither loss of CINP rescues global gene expression by KLF5 overexpression, we compare the effect of siRNA-mediated CINP silencing on K12 cells (TSU-PR1 cell line with stable KLF5 expression).

2020-05-15 | GSE98930 | GEO

Expression of RAW264.7 cells stimulated with RANKL and treated with supernatant from Lactobacillus reuteri

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Profiling of DNA Methylation during RANKL Induced Osteoclastogenesis using Whole-Genome Bisulfite Sequencing

Project description:Purpose: The goals of this study are to describe the DNA methylation profile during RANKL induced osteoclastogenesis. Methods: Primary bone marrow cells were isolated and treated with M-CSF (30ng/mL) to generate bone marrow macrophages (BMMs). BMMs were then used to generate preosteoclast (pOC) stimulated by M-CSF (30ng/mL) together with RANKL (100ng/mL) for 24 h and mature osteoclast (mOC) stimulated by M-CSF (30ng/mL) together with RANKL (100ng/mL) for 72 h or more. DNA methylation profiles of BMM, pOC and mOC were generated by deep sequencing, using Illumina NovaSeq 6000. Results: After acquiring of clean reads, we used Hisat2 for spliced mapping. We mapped about 40 million sequence reads per sample of the WGBS results. Saturation rate analysis was then performed evaluating the gene coverage rate along with read depth increase. The transcripts mapping to difference functional region of the genome was then analyzed. Circos software was used to map the consistency of samples with mouse genome on a chromosomal level. The quantification of transcript intensity was analyzed using RPKM (Reads Per Kb per Million reads). Conclusions: Our study represents the first high-throughput WGBS sequencing data of DNA methylation during osteoclastogenesis.

2020-06-04 | GSE133515 | GEO

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