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The spatio-temporal program of DNA replication is driven by specific epigenetic signatures in human cells

ABSTRACT: We report the application of single-molecule-based sequencing technology for high-throughput profiling of DNA replication origins in K562 mammalian cells. We have optimized a specific method of peak detection adapted to the signal produced by the sequencing of short nascent strands (SNS) that are specific of replication initiation, with the aim to have both a good sensitivity and specificity. We demonstrated the existence of the spatiao-temporal program of DNA replication driven by a specific epigenetic signature.

ORGANISM(S): Homo sapiens

PROVIDER: GSE46189 | GEO | 2014/05/01

SECONDARY ACCESSION(S): PRJNA197531

REPOSITORIES: GEO

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The spatio-temporal program of DNA replication is driven by specific epigenetic signatures in human cells

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of DNA replication origins in K562 mammalian cells. We have optimized a specific method of peak detection adapted to the signal produced by the sequencing of short nascent strands (SNS) that are specific of replication initiation, with the aim to have both a good sensitivity and specificity. We demonstrated the existence of the spatiao-temporal program of DNA replication driven by a specific epigenetic signature. K562 human cells; five samples subjected to SNS-Seq.

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