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Alanine scan of 11 amino acid long novel linear epitope from C.jejuni cj0669 protein [2]

ABSTRACT: A short sequence of 11 amino acids belonging to the cj0669 protein from Campylobacter jejuni NCTC 11168 which was previously identified as potentially immunogenig was analyzed via alanine scanning to narrow down the significant amino acid residues within the sequence.

ORGANISM(S): Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

PROVIDER: GSE46651 | GEO | 2013/05/06

SECONDARY ACCESSION(S): PRJNA201407

REPOSITORIES: GEO

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Alanine scan of 11 amino acid long novel linear epitope from C. jejuni cj0669 protein

2013-04-06 | GSE45556 | GEO

Alanine scan of 11 amino acid long novel linear epitope from C. jejuni cj0669 protein

Project description:A short sequence of 11 amino acids belonging to the cj0669 protein from Campylobacter jejuni NCTC 11168, which was previously identified as potentially immunogenic, was analyzed via alanine scanning to narrow down the significant amino acid residues within the sequence. Twelve peptides, one representing the original sequence and eleven peptides with each residue replaced by alanine in turn, were synthesized on microarrays by JPTs Pepstar Technology. For each microarray, nine replicates for each peptide were spotted. The microarray was separated into three incubation chambers by the ProPlate 3-well module (Grace Biolabs) to allow for incubation with different antibodies in parallel. For specific interactions, rabbit polyclonal IgG to C. jejuni was used, while unspecific binding to the epitope sequence was checked using rabbit polyclonal IgG to Salmonella enterica.

2013-04-06 | E-GEOD-45556 | biostudies-arrayexpress

Alanine scan of 11 amino acid long novel linear epitope from C.jejuni cj0669 protein [2]

Project description:A short sequence of 11 amino acids belonging to the cj0669 protein from Campylobacter jejuni NCTC 11168 which was previously identified as potentially immunogenig was analyzed via alanine scanning to narrow down the significant amino acid residues within the sequence. Twelve peptides, one representing the original sequence and eleven peptides with each residue replaced by alanine in turn, were synthesized on microarrays by JPTs Pepstar Technology. For each microarray, nine replicates for each peptide were spotted. The microarray was seperated into three incubation chambers by the ProPlate 3-well module (Grace Biolabs) to allow for incubation with different antibodies in parallel. For specific interaction rabbit polyclonal IgG to C.jejuni was used, while non-specific binding to the epitope sequence was checked using rabbit polyclonal IgG to H. pylori (Abcam ab20459).

2013-05-06 | E-GEOD-46651 | biostudies-arrayexpress

Global functional impacts of Campylobacter jejuni stress induced by exposure to deoxycholate

Project description:Comparison of the Campylobacter jejuni NCTC 11168 proteome in MH media in the presence and absence of Deoxycholate

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Wild Type 11168 versus LuxS01 Mutant Cultured in MHB and MEM- α media

Project description:Transcriptional profiling of Campylobacter jejuni NCTC 11168 wild type versus LuxS01 mutant strain grown in a minimal culture medium (MEM- α ) versus complex culture medium (MHB).

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RNA-sequencing of Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant

Project description:We report the use of RNA-seq analysis for the determination of RPKM transcript levels in wildtype and fur perR mutant of Campylobacter jejuni NCTC 11168. This allows for comparison of gene expression levels. Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant were grown to late log phase, RNA was purified and used for RNA-sequencing by Illumina HiSeq sequencing

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Project description:We report the use of differential RNA-sequencing for the determination of the primary transcriptome of wildtype Campylobacter jejuni NCTC 11168. This allows for the genome-wide determination of transcription start sites.

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2013-10-10 | E-GEOD-43023 | biostudies-arrayexpress

Transcriptional profiles of Campylobacter jejuni NCTC 11168 to 0.125mg/L erythromycin treatment

Project description:Erythromycin is the drug of choice to treat campylobacteriosis, but resistance to this antibiotic is rising. The adaptive mechanisms employed by Campylobacter jejuni to erythromycin treatment remain unknown. The aim of this study is to determine the molecular basis underlying CampylobacterM-bM-^@M-^Ys immediate response to Ery treatment. The design utilized an available two color microarray slide for the entire transcriptome of Campylobacter jejuni wild type strain NCTC 11168. One hybridizations were performed: sham-treated NCTC 11168 v.s. sub-lethal dose erythromycin treated NCTC 11168. Samples were independently grown and harvested. There were three biological replicates of each sample.

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