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WTAP is a novel oncogenic protein in Acute Myeloid Leukemia

ABSTRACT: Acute myeloid leukemia (AML) continues to have the lowest survival rates of all leukemias. Therefore, new therapeutic strategies are urgently needed to improve clinical outcomes for AML patients. Here, we report a novel role for Wilms’ tumor 1-associated protein (WTAP) in pathogenesis of AML. We have performed RNA-Seq in K562 cells with knockdown of WTAP to ascertain which genes it regulates.

ORGANISM(S): Homo sapiens

PROVIDER: GSE46718 | GEO | 2014/01/22

SECONDARY ACCESSION(S): PRJNA202085

REPOSITORIES: GEO

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WTAP is a novel oncogenic protein in Acute Myeloid Leukemia

Project description:Acute myeloid leukemia (AML) continues to have the lowest survival rates of all leukemias. Therefore, new therapeutic strategies are urgently needed to improve clinical outcomes for AML patients. Here, we report a novel role for Wilms’ tumor 1-associated protein (WTAP) in pathogenesis of AML. We have performed RNA-Seq in K562 cells with knockdown of WTAP to ascertain which genes it regulates. We have 2 replicates of total RNA for K562 cells and 2 replicates with WTAP knocked down

2014-01-22 | E-GEOD-46718 | biostudies-arrayexpress

Binding motif analysis of METTL3，WTAP in METTL3，WTAP overexpressed Human 293T cells

Project description:label the cells overexpressed Myc tagged METTL3 and Flag tagged WTAP with 4-SU, the RNA bound by METT3,WTAP can be got by Myc or Flag IP followed by RNA isolation by using the TRIzol (Invitrogen) reagent by following the company manual.the RNA was then used for library preparation using a TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol.The libraries were sequenced using HiSeq2000 (Illumina) in single-read mode, creating reads with a length of 50 bp. Sequencing chemistry v2 (Illumina) was used and samples were multiplexed in two samples per lane. discovery of the binding motif of METTL3，WTAP in METTL3，WTAP overexpressed Human 293T cells

2014-01-16 | E-GEOD-50580 | biostudies-arrayexpress

The Role of WTAP in Regulating Macrophage-Mediated Osteoimmune Responses and Tissue Regeneration in Periodontitis

Project description:Periodontitis, delineated by the destruction of structures that support teeth, is predominantly propelled by intricate immune responses. Immunomodulatory treatments offer considerable promise for the management of this ailment; however, the modulation of the periodontal immune microenvironment to facilitate tissue regeneration presents a substantial biomedical challenge. Herein, our study investigates the role of Wilms' tumor 1-associating protein (WTAP), a critical m6A methyltransferase, in the immunomodulation of periodontitis and assesses its viability as a therapeutic target. We observed heightened expression of WTAP in macrophages extracted from gingival tissues impacted by periodontitis, with a strong association with M1 polarization. Via loss-of-function experiments, we demonstrated that diminishing WTAP expression precipitates a transition from M1 to M2 macrophage phenotypes amidst inflammatory conditions, thus improving the periodontal immune landscape. Further, RNA sequencing and indirect co-culture assays indicated that suppressing of WTAP expression modulates osteoimmune responses and enhances the osteogenic differentiation of bone marrow stromal cells. The local deployment of adeno-associated virus-shWTAP in murine models of periodontitis robustly validated the therapeutic promise of targeting WTAP in this disease. Collectively, our findings highlight the crucial role of WTAP in orchestrating macrophage-mediated osteoimmune responses and tissue regeneration in periodontitis, proposing novel avenues for immunotherapeutic interventions in its treatment.

2024-05-24 | GSE265968 | GEO

mRNA profiles of WTAP siRNA-treated HUVECs

Project description:To examine the role of WTAP in splicing regulation, we performed high-throughput mRNA sequencing (RNA-seq) on RNA isolated from control, WTAP or Virilizer siRNA-treated HUVECs, yielding 12 million uniquely mapped 75nt pair-end tags from each sample. MapSplice software was used for differential expression and differences in transcript splice junctions . mRNA profiles of control, WTAP or Virilizer siRNA-treated HUVECs were generated by deep sequencing using Illumina GAII.

2014-09-25 | E-GEOD-41180 | biostudies-arrayexpress

siRNA profiling of human HUVEC cells after WTAP knockdown

Project description:The experiment studied the effect of knockdown WTAP in HUVEC. two control samples and two WTAP knockdown samples.

2008-06-12 | E-GEOD-2327 | biostudies-arrayexpress

WTAP-induced change in gene expresssion in cholangiocarcinoma cells

Project description:Knock-down or overexpression of WTAP regulated migration and invasion of cholangiocarcinoma cells in vivo and vitro studies. To investigate the underlying mechanism for WTAP-regulated migration and invasion, the gene expression between the mock cells and the stable cells was compared Total RNA was purified from the mock cells and the stable cells overexpressing WTAP

2015-04-06 | E-GEOD-32134 | biostudies-arrayexpress

METTL3 and WTAP

Project description:METTL3 and WTAP

| PRJNA217977 | ENA

siRNA mediated WTAP knockdown in HUVEC

Project description:The experiment studied the effect of knockdown WTAP in HUVEC. two control samples and two WTAP knockdown samples. Keywords: other

2005-11-30 | GSE2327 | GEO

mRNA profiles of WTAP siRNA-treated HUVECs

2014-09-25 | GSE41180 | GEO

Total RNA-seq of siRNA-mediated WTAP and TPR knockdown

Project description:To identify changes in splicing patterns following WTAP and TPR knockdown, RNA-sequencing was performed on breast cancer cells transfected with siRNAs targeting WTAP or TPR.

2019-12-26 | GSE130873 | GEO

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