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ChIP-seq in MRC5 hIPSCs overexpressing MEG3 RNA.

ABSTRACT: This experiment was designed to detect differences in PRC2 occupancy in a human induced pluripotent cell line (MRC5) naturally deficient in MEG3 RNA after lentiviral overexpression of MEG3.

ORGANISM(S): Homo sapiens

PROVIDER: GSE48515 | GEO | 2013/12/25

SECONDARY ACCESSION(S): PRJNA210418

REPOSITORIES: GEO

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ChIP-seq in MRC5 hIPSCs overexpressing MEG3 RNA.

Project description:This experiment was designed to detect differences in PRC2 occupancy in a human induced pluripotent cell line (MRC5) naturally deficient in MEG3 RNA after lentiviral overexpression of MEG3. MRC5 were transduced with lentiviruses for the overexpression of MEG3 lncRNA or GFP as a control. ChIP-seq was performed with EZH2 and JARID2 antibodies.

2013-12-25 | E-GEOD-48515 | biostudies-arrayexpress

ChIP-seq in MEG3+ and MEG3- hIPSCs

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ChIP-seq in MEG3+ and MEG3- hIPSCs

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overexpression/knockdown MEG3 in C2C12 cell lines

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