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Real-time quantitative PCR analysis of mouse mature CD4SP thymocytes

ABSTRACT: RNA was isolated with the RNeasy kit (Qiagen) from electronically sorted mature CD4SP thymocytes. RNA was reverse transcribed into cDNA by oligo(dT) priming with the QuantiTect Reverse transcription kit (Qiagen). The expression of 84 key genes involved in apoptosis, or programmed cell death was performed with mouse apoptosis RT2ProfilerTM PCR array system (PAMM-012 E; SABiosciences). PCR Array data were analyzed by the Ct data analysis method.

ORGANISM(S): Mus musculus

PROVIDER: GSE49271 | GEO | 2013/07/27

SECONDARY ACCESSION(S): PRJNA213465

REPOSITORIES: GEO

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Real-time quantitative PCR analysis of mouse immature CD4SP thymocytes

Project description:RNA was isolated with the RNeasy kit (Qiagen) from electronically sorted immature CD4SP thymocytes. RNA was reverse transcribed into cDNA by oligo(dT) priming with the QuantiTect Reverse transcription kit (Qiagen). The expression of 84 key genes involved in apoptosis, or programmed cell death was performed with mouse apoptosis RT2ProfilerTM PCR array system (PAMM-012 E; SABiosciences). PCR Array data were analyzed by the Ct data analysis method.

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