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Murine CD4+ and CD8+ T-cells from wildtype and Gfi knockout cells

ABSTRACT: to compare the transcriptomes of naïve CD4+ and CD8+ T-cells from Gfi1-knockout cells with wildtype cells, spleenocytes from wildtype and Gfi-knockout C57Bl/6 mice were isolated, stained with CD44, CD4 and CD8 antibodies and separated by using a FACS Diva (Becton Dickinson). Total RNAs isolated from 2 mice each (approximately 2xE06 cells) were pooled and subjected to microarray analysis on Affymetrix MOE430A_2.0 arrays Keywords: knockout mice

ORGANISM(S): Mus musculus

PROVIDER: GSE4938 | GEO | 2008/07/22

SECONDARY ACCESSION(S): PRJNA104459

REPOSITORIES: GEO

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Transcription profiling of naive CD4+ and CD8+ T-cells from Gfi1-knockout and wild type mice

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