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Genome-wide transcription profile of CpG-activated peritoneal macrophages

ABSTRACT: To compare up-regulation of genes following CpG activation, we performed microarray analysis of activated macrophages from B6 and F1(B6xMOLF) mouse strains. Cells were activated for 0, 2 and 4 hrs with 200nM of type B CpG. Levels of mRNA for many genes differened dramatically between the strains

ORGANISM(S): Mus musculus

PROVIDER: GSE50682 | GEO | 2013/09/07

SECONDARY ACCESSION(S): PRJNA218465

REPOSITORIES: GEO

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Genome-wide transcription profile of CpG-activated peritoneal macrophages

Project description:To compare up-regulation of genes following CpG activation, we performed microarray analysis of activated macrophages from B6 and F1(B6xMOLF) mouse strains. Cells were activated for 0, 2 and 4 hrs with 200nM of type B CpG. Levels of mRNA for many genes differened dramatically between the strains Peritoneal macrophages were elicited from pertoneal cavity of mice 3 days after thioglycollate injection. The cells were plated overnight at a density of 5 million cells per 100 mm dish and activated the next day with 200 nM CpG. Total RNA was isolated with TRIZOL followed by reverse transcrition, fragmentation and labeling accroding the manufacture's (Affymetrix) recommendations

2013-09-07 | E-GEOD-50682 | biostudies-arrayexpress

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Project description:To analyze the difference in gene expression between alleles of F1 hybrids of mouse C57BL6/J (B6) and MSM/Ms (MSM) strains, we sequenced these F1 hybrids of reciprocal crosses. While most genes did show allelic bias, a small number of genes showed either B6- or MSM-biaed expression in both crosses. These allelic differences were associated with the allelic differences in histone acetylation in promoter regions determined by another experiments.

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Gene expression of CD11b+ and CD8+ spleen dendritic cells from NOD and B6 mice after in vivo CpG or PBS stimulation

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Gene expression analysis of Unstimulated follicular B cells and follicular B cells stimulated with IFNa, CpG and CpG plus IFNa.

Project description:We report the grobal gene expression in follicular (FO) B cells stimulated with CpG, IFNa, and CpG plus IFNa for 12 hours prior to the onset of PC differentiation. We found that stimulation with CpG plus IFNa upregulates metabolic processes compared to stimulation with CpG alone. This study highlight the pivotal role of IFNa in determining the fate of PC differentiation of TLR9-activated FO B cells.

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Genomic DNA copy number aberration in chromosome 18 of small intestinal tumors in B6/B6-Chr18MSM-F1 ApcMin/+ mice irradiated with ionizing radiation

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Gene expression profile in peritoneal macrophage stimulated with B-DNA or CpG-B

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