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Expression Profiling of MAP Kinase-Mediated Meiotic Progression in C. elegans

ABSTRACT: A unique allele of MAP kinase, mpk-1(ga111), displays a reversible, temperature-sensitive, tissue-specific defect in progression through meiotic prophase I. We performed gene expression profiling on mpk-1(ga111) animals at multiple times after shift to permissive temperature to identify candidate downstream effectors of MPK-1 signaling in the germline. This analysis delineated a cohort of genes whose expression requires MPK-1 (MAP kinase) signaling in germ cells in the pachytene stage of meiosis I. Keywords: Timecourse of C. elegans mpk-1 mutants

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE5069 | GEO | 2006/08/01

SECONDARY ACCESSION(S): PRJNA96705

REPOSITORIES: GEO

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Expression Profiling of MAP Kinase-Mediated Meiotic Progression in C. elegans

Project description:A unique allele of MAP kinase, mpk-1(ga111), displays a reversible, temperature-sensitive, tissue-specific defect in progression through meiotic prophase I. We performed gene expression profiling on mpk-1(ga111) animals at multiple times after shift to permissive temperature to identify candidate downstream effectors of MPK-1 signaling in the germline. This analysis delineated a cohort of genes whose expression requires MPK-1 (MAP kinase) signaling in germ cells in the pachytene stage of meiosis I. We analyzed six timepoints after MAP kinase mpk-1(ga111) mutants were shifted to a temperature permissive for meiotic progression. Each timepoint was sampled in triplicate. Control (mpk-1+) animals were sampled in triplicate at the beginning and end of the time series. All samples were compared to a common reference sample made of a mixture of both control samples.

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