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Expression Profiling of MAP Kinase-Mediated Meiotic Progression in C. elegans


ABSTRACT: A unique allele of MAP kinase, mpk-1(ga111), displays a reversible, temperature-sensitive, tissue-specific defect in progression through meiotic prophase I. We performed gene expression profiling on mpk-1(ga111) animals at multiple times after shift to permissive temperature to identify candidate downstream effectors of MPK-1 signaling in the germline. This analysis delineated a cohort of genes whose expression requires MPK-1 (MAP kinase) signaling in germ cells in the pachytene stage of meiosis I. We analyzed six timepoints after MAP kinase mpk-1(ga111) mutants were shifted to a temperature permissive for meiotic progression. Each timepoint was sampled in triplicate. Control (mpk-1+) animals were sampled in triplicate at the beginning and end of the time series. All samples were compared to a common reference sample made of a mixture of both control samples.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Valerie Reinke 

PROVIDER: E-GEOD-5069 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Expression profiling of MAP kinase-mediated meiotic progression in Caenorhabditis elegans.

Leacock Stefanie W SW   Reinke Valerie V  

PLoS genetics 20061101 11


The LET-60 (Ras)/LIN-45 (Raf)/MPK-1 (MAP kinase) signaling pathway plays a key role in the development of multiple tissues in Caenorhabditis elegans. For the most part, the identities of the downstream genes that act as the ultimate effectors of MPK-1 signaling have remained elusive. A unique allele of mpk-1, ga111, displays a reversible, temperature-sensitive, tissue-specific defect in progression through meiotic prophase I. We performed gene expression profiling on mpk-1(ga111) animals to iden  ...[more]

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