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PARK2 knockdown in 2 glioma cell lines

ABSTRACT: The PARK2 gene was knocked down using 2 independent siRNAs in SNB19 and SF539 cell lines A non-targeted scramble siRNA was used as the control.

ORGANISM(S): Homo sapiens

PROVIDER: GSE50864 | GEO | 2014/04/11

SECONDARY ACCESSION(S): PRJNA219200

REPOSITORIES: GEO

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PARK2 knockdown in 2 glioma cell lines

Project description:The PARK2 gene was knocked down using 2 independent siRNAs in SNB19 and SF539 cell lines A non-targeted scramble siRNA was used as the control. Scramble, PARK2 siRNA#1 or PARK2 siRNA#2 was transfected into each cell line, in duplicate, and RNA analyzed using the Affymetrix U133A 2.0 platform.

2014-04-11 | E-GEOD-50864 | biostudies-arrayexpress

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Project description:PARK2 (PARKIN) is an E3 ubiquitin ligase whose dysfunction has been associated with the progression of Parkinsonism and human malignancies, and its role in cancer remains to be explored. In this study, we investigated its role in glioma. We used microarrays to detail the global programme of gene expression upon PARK2 overexpression in U251 cells U251 cells with PARK2 overexpression or control (GFP) were subjected for RNA extraction and hybridization on Affymetrix microarrays.

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Expression data from PARK2 overexpression in U251 cells

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