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Gene expression profile of hematopoietic stem cells (CD34+ cells) with or without nanoparticles

ABSTRACT: To understand the the effect of poly(lactic-co-glycolic acid) nanoparticles (NPs) encapsulating a fluorine contrast agent on hematopoietic stem cells (CD34+ fraction of umbilical cord blood mononuclear cells), we have employed whole genome microarray expr

ORGANISM(S): Homo sapiens

PROVIDER: GSE51421 | GEO | 2014/06/01

SECONDARY ACCESSION(S): PRJNA223001

REPOSITORIES: GEO

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Gene expression profile of hematopoietic stem cells (CD34+ cells) with or without nanoparticles

Project description:To understand the the effect of poly(lactic-co-glycolic acid) nanoparticles (NPs) encapsulating a fluorine contrast agent on hematopoietic stem cells (CD34+ fraction of umbilical cord blood mononuclear cells), we have employed whole genome microarray expr The gene expression in human CD34+ cells was measured at 24 h and 7 d after incubation with nanoparticles. Three independent experiments were performed at each time.

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Project description:The mortality of patients suffering from acute myocardial infarction (AMI) is linearly related to the infarct size. As regeneration of cardiomyocytes from cardiac progenitor cells is minimal in the mammalian adult heart, we have explored a new therapeutic approach which leverages the capacity of nanomaterials to release chemicals over time to promote myocardial protection and infarct size reduction. Initial screening identified two chemicals, FGF1 and CHIR99021 (a Wnt1 agonist/GSK-3 antagonist) which synergistically enhance cardiomyocyte cell cycle in vitro. Poly-lactic-co-glycolic acid (PLGA) nanoparticles (NP) formulated with CHIR99021 and FGF1 (CHIR+FGF1-NPs) provided an effective slow release system for up to 4 weeks. Intramyocardial injection of CHIR+FGF1-NPs enabled myocardial protection via reducing infarct size by 20-30% in mouse or pig models of postinfarction LV remodeling. This LV structural improvement was accompanied by a significant preservation of cardiac contractile function. Further investigation revealed that CHIR+FGF1-NPs resulted in a significant reduction of cardiomyocyte apoptosis and increase of angiogenesis. Thus, using a combination of chemicals and a NP-based prolonged release system that work synergistically, this study demonstrates a novel therapy for LV infarct size reduction in hearts with acute myocardial infarction.

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Global genetic impact of surface-modified gold nanoparticles on two model human cell lines

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