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Microarray analysis of miRNAs involved in Porcine Alveolar Macrophages at the early stage of porcine reproductive and respiratory syndrome virus infection [LCS_miRPig_18]

ABSTRACT: By analyzing the changes of miRNAs of piglet PAMs at different time points after PRRSV infection,we can find porcine miRNAs associated with viral infection and possible target sites in both PRRSV and pig genome and their roles in protection against viral infections. Then these targets will screened by bioinformatics methods to find the susceptibility genes, resistance genes and immune related genes, and formed molecular biology network of miRNAs at gene and protein level .

ORGANISM(S): Sus scrofa

PROVIDER: GSE52010 | GEO | 2013/11/01

SECONDARY ACCESSION(S): PRJNA226104

REPOSITORIES: GEO

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Microarray analysis of miRNAs involved in Porcine Alveolar Macrophages at the early stage of porcine reproductive and respiratory syndrome virus infection [LCS_miRPig_17]

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Microarray analysis of miRNAs involved in Porcine Alveolar Macrophages at the early stage of porcine reproductive and respiratory syndrome virus infection [LCS_miRPig_18]

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2013-11-01 | E-GEOD-52010 | biostudies-arrayexpress

Microarray analysis of miRNAs involved in Porcine Alveolar Macrophages at the early stage of porcine reproductive and respiratory syndrome virus infection [LCS_miRPig_17]

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Transcriptome analysis of the mRNAs and lncRNAs in porcine alveolar macrophages in response to porcine reproductive and respiratory syndrome virus or IFN-a at different time points

Project description:Purpose:We performed next-generation RNA sequencing and a comprehensive bioinformatics analyses to characterize the transcriptome landscapes, including mRNAs and long noncoding RNAs (lncRNAs) in PRRSV-infected PAMs or IFN-stimulated PAMs at different time points. Conclusions: We showed that PRRSV infection displays different transcriptome profiles of PAMs, and the aberrant function of PAMs may be reflected by significant down-regulation of essential genes at the transcriptome levels. In addition, we discovered a great amount of differentially expressed lncRNAs, including lncRNA XR_297549.1, which is highly expressed and predicted to both cis- and trans-regulate its neighboring gene PTGS2.In addition, In order to expand our understanding of these IFN-induced non-coding transcripts, especially long non-coding RNAs (lncRNAs), we performed next-generation RNA sequencing and a comprehensive bioinformatics analysis of porcine alveolar macrophages (PAMs) stimulated with pig-IFN-? for 6h and 12h, respectively. A great amount of differentially expressed mRNAs and lncRNAs were identified, and several lncRNAs are of great interest for further investigation as their potential role in regulation of the well-characterized antiviral genes, such as OAS1, PML and ISG15.

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2008-09-01 | E-GEOD-10346 | biostudies-arrayexpress

RNA-sequencing analysis of gilt blood gene expression during Porcine Reproductive and Respiratory Syndrom Virus infection

Project description:Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection of pregnant females causes fetal death and increased piglet mortality, but there is substantial variation in the extent of reproductive pathology between individual dams. This study used RNA-sequencing to characterize the whole blood transcriptional response to PRRSV in pregnant gilts during the first week of infection, and attempted to identify gene expression signatures associated with a low or high level of fetal mortality at necropsy, 21 days post-inoculation. RNA-sequencing experiment compared whole blood gene expression in two groups of gilts: a low fetal mortality group (LFM) and a high fetal mortality (HFM) group (n=8 per group). Two sets of contrasts were perfromed. First, a time-course experiment in which gene expression at day 2 (D2) post-inoculation was compared to day 0 (D0-just prior to inoculation) and expression at day 6 post-inoculation was compared to D2. Second, a comparison of gene expression between LFM and HFM groups at each of the three time-points (D0, D2, and D6).

2016-05-17 | E-GEOD-75304 | biostudies-arrayexpress

Transcriptome Analysis of Porcine Immune Cells Stimulated by Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Caesalpinia sappan Extract

Project description:The current level of knowledge on transcriptome responses triggered by Caesalpinia sappan (CS) extract in porcine peripheral blood mononuclear cells (PBMCs) after porcine reproductive and respiratory syndrome virus (PRRSV) infection is limited. Therefore, in the present study, we aimed to detect significant genes and pathways involved in CS extract supplementation responsiveness of PBMCs after PRRSV infection. RNA sequencing was conducted on PBMCs, which were isolated from six weaned piglets. The resultant transcriptional responses were examined by mRNA sequencing. Differential expression analysis identified 263 and 274 differentially expressed genes (DEGs) between the PRRSV and CTRL groups, and the PRRSV+CS and CTRL groups, respectively. Among these, ZNF646 and KAT5 emerged as the most promising candidate genes, potentially influencing the interaction between PRRSV-infected PBMCs and CS extract supplementation through the regulation of gene networks and cellular homeostasis during stress. Two pathways were detected to be associated with CS extract supplementation responsiveness: the cellular response to stress pathway and the NF-kB signaling pathway. Consequently, our study reveals a novel mechanism underlying cellular stress response and the NF-κB signaling pathway in PRRSV-infected PBMCs, and identifies a potential application of CS extract for activating the NF-κB signaling pathway. In conclusion, by supplementing CS extract in PBMC cells infected with PRRSV, we found that CS extract modulates PRRSV infection by inducing cellular stress, which is regulated by the NF-κB signaling pathway. This induced stress creates an adverse environment for PRRSV survival. This study contributes to a deeper understanding of the therapeutic targets and pathogenesis of PRRSV infection. Importantly, our results demonstrate that CS extract has the potential to be a candidate for modulating PRRSV infection.

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