Expression profile of select inflammation-related genes after miR-466l overexpression or knockdown in human macrophages
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ABSTRACT: Human Peripheral blood mononuclear cells (PBMC) were isolated from healthy human whole blood by density gradient centrifugation using Histopaque®-1077 and cultured in RPMI (containing 10% FBS) with 10 ng/ml human recombinant GM-CSF at 37 °C for 7 days. These cells are confirmed as human macrophages. These macrophages were transfected with vector control, miR-466l,anti-miR-negative or anti-miR-466l (37 °C, 72h). After transfection, total RNA was extracted for reverse transcription. The product cDNA was collected for real-time PCR with respective primers.
ORGANISM(S): Homo sapiens
PROVIDER: GSE52174 | GEO | 2013/11/08
SECONDARY ACCESSION(S): PRJNA227212
REPOSITORIES: GEO
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