Real-time quantitative PCR analysis of human leukemic B-cells.
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ABSTRACT: Fresh blood samples were collected from 10 CLL (chronic lymphocytic leukemia) previously untreated patients before and after 2 weeks of the first cycle of CCR (cladribine, cyclophosphamide, rituximab) treatment. Peripheral blood mononuclear cells (PBMNCs) were separated from EDTA blood by layering on the Histopaque 1077 and centrifuging on a density gradient. Mean B-cell CD19+ purity was ≥95% as measured by flow cytometry (FC). Microarray analysis was performed using 0.5 microgram of RNA transcribed to cDNA. Prepared cDNA samples were subjected to RT-PCR in duplicate in the TaqMan 7900HT Sequence Detection System (Applied Biosystems).
ORGANISM(S): Homo sapiens
PROVIDER: GSE33925 | GEO | 2013/12/31
SECONDARY ACCESSION(S): PRJNA148223
REPOSITORIES: GEO
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