Transcriptomics

Dataset Information

0

Cigarette smoke-induced iBALT mediates macrophage activation in a B cell-dependent manner in COPD


ABSTRACT: Chronic obstructive pulmonary disease (COPD) is characterized by a progressive decline in lung function, caused by exposure to exogenous particles, mainly cigarette smoke (CS). COPD pathogenesis is initiated and perpetuated by an abnormal CS-induced inflammatory response of the lungs, involving both innate and adaptive immunity. Specifically, B cells organized in iBALT structures, as well as macrophages, accumulate in the lungs and contribute to CS-induced emphysema, but the mechanisms thereof remain unclear. Here, we demonstrate that B cell-deficient mice are significantly protected against CS-induced emphysema. Chronic CS exposure led to increased lung compliance, total lung capacity, and mean linear chord length in WT, but not B cell-deficient mice, associated with an increased size and number of iBALT structures. The increased accumulation of macrophages around iBALT and in emphysematous alveolar areas in CS-exposed WT mice coincided with upregulated MMP12 expression. In vitro co-culture experiments using B cells and macrophages demonstrated that B cell-derived IL-10 drives macrophage activation and MMP12 upregulation. In summary, B cell function in iBALT formation in CS-induced emphysema provides a new innovative mechanism, which could be explored as a target for therapeutic intervention in COPD patients.

ORGANISM(S): Mus musculus

PROVIDER: GSE52509 | GEO | 2014/12/04

SECONDARY ACCESSION(S): PRJNA229140

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-12-04 | E-GEOD-52509 | biostudies-arrayexpress
2012-09-11 | E-GEOD-27597 | biostudies-arrayexpress
2015-11-30 | PXD002530 | Pride
2012-09-11 | GSE27597 | GEO
2013-12-31 | E-GEOD-49881 | biostudies-arrayexpress
2017-08-05 | E-MTAB-5280 | biostudies-arrayexpress
2004-08-08 | GSE1650 | GEO
2013-12-31 | GSE49881 | GEO
2007-08-13 | E-GEOD-1650 | biostudies-arrayexpress
2015-12-01 | E-MTAB-3681 | biostudies-arrayexpress