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Highly parallel identification of sequence preferences for eukaryotic C2H2 zinc finger domains using a bacterial 1-hybrid assay

ABSTRACT: The largest and most diverse class of eukaryotic transcription factors contain Cys2-His2 zinc fingers (C2H2-ZFs), each of which typically binds a DNA nucleotide triplet within a larger binding site. Frequent recombination and diversification of their DNA-contacting residues suggests that these zinc fingers play a prevalent role in adaptive evolution. Very little is known about the function and evolution of the vast majority of C2H2-ZFs, including whether they even bind DNA. Using the bacterial 1-hybrid (B1H) system, we determined DNA-binding motifs for thousands of individual natural C2H2-ZFs, and correlated them with the C2H2-ZF specificity residues.

ORGANISM(S): synthetic construct

PROVIDER: GSE52521 | GEO | 2015/02/17

SECONDARY ACCESSION(S): PRJNA229157

REPOSITORIES: GEO

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Evaluation of DNA-binding preferences of C2H2 zinc finger domains by PBM

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