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Rat insulin-producing INS-1E exposed to cytokines IL-1β and IFN-γ

ABSTRACT: In the context of T1 Diabetes, pro-inflammatory cytokines IL-1β and IFN-γ are known to contribute to β-cell apoptosis; The measurement of mRNA expression following β-cell exposure to these cytokines gives a picture of the changes in gene expression characterizing the path to β-cell dysfunction and death.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE53453 | GEO | 2013/12/19

SECONDARY ACCESSION(S): PRJNA232041

REPOSITORIES: GEO

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Rat insulin-producing INS-1E exposed to cytokines IL-1β and IFN-γ

Project description:In the context of T1 Diabetes, pro-inflammatory cytokines IL-1β and IFN-γ are known to contribute to β-cell apoptosis; The measurement of mRNA expression following β-cell exposure to these cytokines gives a picture of the changes in gene expression characterizing the path to β-cell dysfunction and death. INS1 cell lines were cultured in medium with or without IL-1β and IFN-γ. The samples were collected at various time points for profiling with Affymetrix Rat ST arrays. These experiments were performed on two separate occasions.

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