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Microarray analysis of beta-catenin regulated target genes in SW480 colon cancer cells

ABSTRACT: Deregulation of the canonical Wnt/beta-catenin pathway is one of the earliest events in the pathogenesis of colon cancer. Mutations in APC or CTNNB1 are frequent in colon cancer and cause aberrant stabilization of beta-catenin, which activates Wnt target genes by binding to chromatin via TCF/LEF transcription factors. In a comprehensive study, we conducted an integrative analysis of genome-wide chromatin occupancy of beta-catenin by chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) along with gene expression profiling changes resulting from RNAi-mediated knockdown of beta-catenin in colon cancer cells. This experiment series represents the gene expression changes detected by microarray as a result of CTNNB1 perturbation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE53656 | GEO | 2013/12/27

SECONDARY ACCESSION(S): PRJNA232592

REPOSITORIES: GEO

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Identification of beta-catenin binding regions in SW480 cells

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