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Elevated MAOA Expression Enhances Tumor Growth In-vivo and Alters Transcriptional Profiles

ABSTRACT: Subcutaneous xenografts were derived utilizing either PC3 cells engineered to stably overexpress MAOA or empty vector controls in mice receiving docetaxel treatment. Tumor gene expression was profiled using Agilent gene expression microarrays.

ORGANISM(S): Homo sapiens

PROVIDER: GSE53944 | GEO | 2014/07/03

SECONDARY ACCESSION(S): PRJNA234007

REPOSITORIES: GEO

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Elevated MAOA Expression Enhances Tumor Growth In-vivo and Alters Transcriptional Profiles

Project description:Subcutaneous xenografts were derived utilizing either PC3 cells engineered to stably overexpress MAOA or empty vector controls in mice receiving docetaxel treatment. Tumor gene expression was profiled using Agilent gene expression microarrays. Custom Agilent 44K whole human genome expression oligonucleotide microarrays were used to profile subcutaneous xenograft tumors derived utilizing either PC3 cells engineered to stably overexpress MAOA or empty vector controls in mice receiving docetaxel treatment. Total RNA was isolated and amplified prior to hybridization against a common reference pool of prostate tumor cell lines.

2014-07-03 | E-GEOD-53944 | biostudies-arrayexpress

A synonymous mutation of rs1137070 cause the Mice Maoa gene transcription and translation to decrease

Project description:Monoamine oxidase-A (MAOA) EcoRV polymorphism (rs1137070) is a unique synonymous mutation (c.1409 T >C) of the Maoa gene that plays a role in the expression and Maoa function. This study explored the relationship between the mouse Maoa rs1137070 and the differences in Maoa gene expression. The mouse with the CC genotype of rs1137070 had a lower odds ratio of 2.44 for the Maoa level compared with the T carriers. The TT wild genotype of Maoa had elevated mRNA expression and a longer half-life, significant expression and structure differences of genotype were explored. In addition, the Maoa with different aspartic acid synonymous codons affect Maoa expression and enzyme activity that underlines the association between rs1137070 and Maoa. A dual-luciferase reporter assay confirmed that GAC was more efficient than GAT binding. On the other hand, the synonymous mutation altered the Maoa gene expression in individual mice and RNA pull-down assay suggested that it could affect the interaction with RNA binding protein. The result illustrated that synonymous mutation can indeed regulate the downregulation of gene expression, leading to changes in Maoa function and neurological-related diseases.

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Silenced LINC01963 enhanced the chemosensitivity of prostate cancer cells to docetaxel by sponging miR-216b-5p to inhibit TrkB protein level

Project description:Long noncoding RNAs (lncRNAs) affect docetaxel chemosensitivity. However, the biological role and regulatory mechanisms of lncRNAs in docetaxel-resistant prostate cancer remain unclear. Differences in lncRNAs were evaluated by lncRNA sequencing and evaluated using quantitative real-time polymerase chain reaction, and TrkB expression was measured through Western blot analysis. Proliferation was measured using the MTS, while apoptosis and cell cycle were measured using flow cytometry. Additionally, migration and invasion were measured using transwell assays. Forty-eight female BALB/c nude mice were used for subcutaneous tumorigenicity and lung metastasis assays. We found that LINC01963 was overexpressed in the PC3-DR cells. LINC01963 silencing enhanced the chemosensitivity of PC3-DR to docetaxel and inhibited tumorigenicity and lung metastasis, while LINC01963 overexpression enhanced the chemoresistance of PC3 cells to docetaxel. It was found that LINC01963 sponges miR-216b-5p. The miR-216b-5p inhibitor reversed the suppressive effect of sh-LINC01963 on PC3-DR cell proliferation, migration, and invasion. Furthermore, miR-216b-5p can bind to the 3’-UTR of NTRK2 and inhibit TrkB protein levels. TrkB enhances docetaxel resistance in prostate cancer and reverses the effects of LINC01963 silencing and miR-216b-5p overexpression. In conclusion, silencing LINC01963 enhanced the chemosensitivity of PC3-DR to docetaxel by sponging miR-216b-5p to inhibit TrkB protein levels.

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Genome-wide analysis of MAOA on Norepinphrine induced HCC gene expression

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Transcriptional analysis of the effect of monoamine oxidase A (MAOA) in human prostate cancer enzalutamide (ENZ)-resistant C4-2B (C4-2B ENZR) and LNCaP cells

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Project description:Study aimed at evaluating differences in Du145, PC3 and LNCaP human prostate cancer cells treated with ATX-101 and docetaxel for 24h.

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Expression data from xenograft in BALB/c 6-wk-old nude mice with PC3 prostate cancer cells stably expressing PML or a vector control after treatment of the mice with palbociclib

Project description:Expression data from xenograft in BALB/c 6-wk-old nude mice with PC3 prostate cancer cells stably expressing PML or a vector control after treatment of the mice with palbociclib (100mg/kg/day diluted in sodium lactate 50mM pH4 given by gavage) during 5 consecutive days PML plays a role in the senescence tumor suppressor response of normal cells to oncogenic stress. PML also mediates therapy-induced senescence in acute promyelocytic leukemia cells after treatment with retinoic acid. However, many tumor cell lines fail to engage a complete senescence response to PML activation. Interestingly, in vivo and in vitro combination of PML overexpression with CDK inhibition induce senescence in PC3 prostate cancer cells. We used microarray to characterize the gene expression profile of PC3 stably expressing PML or a control vector after subcutaneous injection in BALB/c 6-wk-old nude mice and treatment of the mice with palbociclib (100mg/kg/day) during 5 consecutive days.. PC3 cells were infected with a retroviral vector that express PML or an empty vector before subcutaneous injection of 10^6 cells in both flanks of BALB/c 6-wk-old nude mice. Then, mice were treated for 5 consecutive days with palbociclib (100mg/kg/day diluted in sodium lactate 50mM pH4 given by gavage)

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