Genomics

Dataset Information

0

TOP1α functions on nucleosome density


ABSTRACT: DNA topoisomerases release supercoils in DNA introduced during replication or transcription. How DNA topoisomerases impact transcription in the context of eukaryotic chromatin is poorly understood. In this study, using a floral stem cell model in Arabidopsis, we uncovered a role of TOP1α in Polycomb Group (PcG) protein-mediated histone lysine 27 trimethylation at, and transcriptional repression of, the stem cell maintenance gene WUSCHEL (WUS). The strong genetic interactions between a top1α mutant and mutations in PcG genes and the overwhelming enrichment of PcG targets among genes affected in expression in the top1a mutant revealed a role of TOP1α in PcG-mediated regulation. Intriguingly, not only the repression of some PcG target genes but also the expression of others requires TOP1α. The mechanism that unifies the opposing effects of TOP1α on PcG target genes appears to lie in its role in decreasing nucleosome density. Genome-wide nucleosome mapping shows that TOP1α is required for the depletion of nucleosomes at regulatory regions of genes, which probably allows the binding of factors that either recruit PcG, as we show for AGAMOUS at the WUS locus, or counteract PcG-mediated regulation. This study uncovers a strong and previously unknown connection between TOP1α and PcG.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE53950 | GEO | 2014/10/14

SECONDARY ACCESSION(S): PRJNA234027

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-10-14 | E-GEOD-42403 | biostudies-arrayexpress
2014-10-14 | E-GEOD-53950 | biostudies-arrayexpress
2014-10-14 | GSE42403 | GEO
2011-08-01 | GSE22626 | GEO
2013-10-31 | E-GEOD-47795 | biostudies-arrayexpress
2013-10-31 | GSE47795 | GEO
2013-02-16 | E-GEOD-43751 | biostudies-arrayexpress
2013-02-16 | GSE43751 | GEO
2022-02-02 | GSE135978 | GEO
2012-08-01 | GSE22809 | GEO