Unknown,Transcriptomics,Genomics,Proteomics

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TOP1M-NM-1 functions on nucleosome density


ABSTRACT: DNA topoisomerases release supercoils in DNA introduced during replication or transcription. How DNA topoisomerases impact transcription in the context of eukaryotic chromatin is poorly understood. In this study, using a floral stem cell model in Arabidopsis, we uncovered a role of TOP1M-NM-1 in Polycomb Group (PcG) protein-mediated histone lysine 27 trimethylation at, and transcriptional repression of, the stem cell maintenance gene WUSCHEL (WUS). The strong genetic interactions between a top1M-NM-1 mutant and mutations in PcG genes and the overwhelming enrichment of PcG targets among genes affected in expression in the top1a mutant revealed a role of TOP1M-NM-1 in PcG-mediated regulation. Intriguingly, not only the repression of some PcG target genes but also the expression of others requires TOP1M-NM-1. The mechanism that unifies the opposing effects of TOP1M-NM-1 on PcG target genes appears to lie in its role in decreasing nucleosome density. Genome-wide nucleosome mapping shows that TOP1M-NM-1 is required for the depletion of nucleosomes at regulatory regions of genes, which probably allows the binding of factors that either recruit PcG, as we show for AGAMOUS at the WUS locus, or counteract PcG-mediated regulation. This study uncovers a strong and previously unknown connection between TOP1M-NM-1 and PcG. 4 samples in total, two replicates from Ler and top1a-2 plants

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Lei Gao 

PROVIDER: E-GEOD-53950 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

DNA topoisomerase I affects polycomb group protein-mediated epigenetic regulation and plant development by altering nucleosome distribution in Arabidopsis.

Liu Xigang X   Gao Lei L   Dinh Thanh Theresa TT   Shi Ting T   Li Dongming D   Wang Ruozhong R   Guo Lin L   Xiao Langtao L   Chen Xuemei X  

The Plant cell 20140728 7


It has been perplexing that DNA topoisomerases, enzymes that release DNA supercoils, play specific roles in development. In this study, using a floral stem cell model in Arabidopsis thaliana, we uncovered a role for TOPOISOMERASE1α (TOP1α) in Polycomb Group (PcG) protein-mediated histone 3 lysine 27 trimethylation (H3K27me3) at, and transcriptional repression of, the stem cell maintenance gene WUSCHEL (WUS). We demonstrated that H3K27me3 deposition at other PcG targets also requires TOP1α. Intri  ...[more]

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