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Auratus goldfish genome project: Muscimol experiment (6 hour)

ABSTRACT: A goldfish brain enriched cDNA combined with a carp microarray (Gracey et al., 2004). Female goldfish were i.p. injected with 1 ug/g muscimol once and after 6 hours, telencephalon dissected and used in microarray analysis. More details on microarray hybridizations and technique used in our laboratory in Martyniuk et al. Gene expression profiling in the brain of male goldfish exposed to 17α-EE2(paper under review) Keywords: muscimol, GABA, goldfish, brain

ORGANISM(S): Cyprinus carpio Carassius auratus

PROVIDER: GSE5419 | GEO | 2006/08/04

SECONDARY ACCESSION(S): PRJNA96073

REPOSITORIES: GEO

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Auratus goldfish genome project: Muscimol experiment (6 hour)

Project description:A goldfish brain enriched cDNA combined with a carp microarray (Gracey et al., 2004). Female goldfish were i.p. injected with 1 ug/g muscimol once and after 6 hours, telencephalon dissected and used in microarray analysis. More details on microarray hybridizations and technique used in our laboratory in Martyniuk et al. Gene expression profiling in the brain of male goldfish exposed to 17Î±-EE2(paper under review) Keywords: muscimol, GABA, goldfish, brain 4 arrays using a common control pool and 3 treatment samples. Slides 16, 22, 33, and 50 Slides 22 and 33 are dye swap.

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Auratus: Goldfish genome project

Project description:Muscimol i.p. injection 6 hour treatment August female fish 2-3 years of age Keywords: single pharmacoligical injection of GABA agonist muscimol; effects in neuroendocrine brain

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Auratus goldfish genomics project - E2 effect on male goldfish hypothalamus

Project description:Adult male goldfish, exposed to estrogen (E2) implant, 1 day exposure See the following for further details; Marlatt VL, Martyniuk, CJ, Zhang, D, Xiong, H, Xia, X, Trudeau, VL, Moon, T. Tissue-specific auto-regulation of estrogen receptor subtypes and transcript profiling of estrogen-responsive genes in the neuroendocrine brain of male goldfish (Carassius auratus) exposed to 17beta-estradiol. J. Mol. Endocrinol. (accepted). Keywords: Estrogen effect on male goldfish hypothalamus, microarray

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Auratus: Goldfish genome project

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Secretoneurin: A new hormone? Auratus: The goldfish environmental genomics project

Project description:The objectives of this study were to examine the effect of secretoneurin (SN) on pituitary gene expression. A mixture of goldfish pituitaries from both males and females were dispersed using Typsin type II and DNase II enzymes. The cells were plated in 24-well culture plates at a density of ~2.5 x 105 cells/well. After at least 12-hr plating period, cells were washed with testing medium and pre-incubated for 1 hr to stabilize basal LH secretion. Dispersed pituitary cells were treated with 10 nM gfSN for 12 hours in static incubation after which the cells were carefully washed by 1X PBS. Total RNA was isolated by utilizing RNeasy Micro Kit (QIAGEN GmbH, Hilden, Germany) following the company’s standard protocol. RNA concentrations were determined using the NanoDrop ND-100 spectrophotometer (Thermo Scientific, Wilmington, DE). cDNA synthesis, cDNA purification and microarray slide hybridization followed our validated and established microarray protocol (Martyniuk et al., 2006; Zhang et al., 2009; Popesku et al., 2010).

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