Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Auratus: Goldfish genome project

ABSTRACT: Muscimol i.p. injection 6 hour treatment August female fish 2-3 years of age Keywords: single pharmacoligical injection of GABA agonist muscimol; effects in neuroendocrine brain Slide 2 and 31 are dye swap 3-4 hypothalami per sample

ORGANISM(S): Carassius auratus

SUBMITTER: Vance Trudeau

PROVIDER: E-GEOD-5768 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Auratus goldfish genome project: effects of fluoxetine on female goldfish

Project description:A goldfish brain enriched cDNA combined with a carp microarray (Gracey et al., 2004). Female adult goldish were treated twice a week for 17 days, for a total of five injections. Each of the injections consisted of 5 Î¼g fluoxetine/g of body weight with an injection volume of 1Î¼l/g. The telencephalon was tissue used. Keywords: i.p.injection of muscimol, 6 hours, pharmacological 4 slides (n=3 treatment samples and common control pool) slide 19 = treatment sample 1 slide 30 = treatment sample 2 slide 36 = treatment sample 3 (dye swap) slide 39 = treatment sample 3 (dye swap)

2010-07-01 | E-GEOD-5420 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: sulpiride in the telencephalon

Project description:Sexually regressing female goldfish were administered 2 i.p. injections: 1) 6 ug/g sulpiride in 0.1% DMSO and 0.6% saline, and; 2) 0.6% saline Tissues were harvested 5h post-injection. A common reference design was used: 4 independent groups of telencephali from sexually regressing female goldfish treated with 6 ug/g sulpiride hybridized against a common vehicle control. Slides 12, 26, 37, 48

2013-03-20 | E-GEOD-15705 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: sulpiride in the hypothalamus

Project description:Sexually regressing female goldfish were administered 2 i.p. injections: 1) 6 ug/g sulpiride in 0.1% DMSO and 0.6% saline, and; 2) 0.6% saline Tissues were harvested 5h post-injection. A common reference design was used: 4 independent groups of hypothalami from sexually mature female goldfish treated with 6 ug/g sulpiride hybridized against a common vehicle control. Slides 6, 15, 28, 44.

2013-03-20 | E-GEOD-15706 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: SCH 23390 in the hypothalamus

Project description:Sexually regressing female goldfish were administered 2 i.p. injections: 1) 40 ug/g SCH 23390 in 0.1% DMSO and 0.6% saline, and; 2) 0.6% saline Tissues were harvested 5h post-injection. A common reference design was used: 4 independent groups of telencephali from sexually regressing female goldfish treated with 40 ug/g SCH 23390 hybridized against a common vehicle control. Slides 16, 24, 30, 35

2013-03-20 | E-GEOD-15708 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: SCH 23390 in the telencephalon

2013-03-20 | E-GEOD-15707 | biostudies-arrayexpress

Auratus goldfish genome project: Muscimol experiment (6 hour)

Project description:A goldfish brain enriched cDNA combined with a carp microarray (Gracey et al., 2004). Female goldfish were i.p. injected with 1 ug/g muscimol once and after 6 hours, telencephalon dissected and used in microarray analysis. More details on microarray hybridizations and technique used in our laboratory in Martyniuk et al. Gene expression profiling in the brain of male goldfish exposed to 17Î±-EE2(paper under review) Keywords: muscimol, GABA, goldfish, brain 4 arrays using a common control pool and 3 treatment samples. Slides 16, 22, 33, and 50 Slides 22 and 33 are dye swap.

2010-07-01 | E-GEOD-5419 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: dopamine depletion in hypothalamus and telencephalon

Project description:Sexually mature (mid-May) adult female goldfish received a single injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP; 50 ug/g body weight at 5 uL/g body weight) or 0.6% saline on day 0 and a single injection of alpha-methyl-p-tyrosine (aMPT; 240 ug/g body weight at 5 uL/g body weight) or 0.6% saline on Day 5 with the goal of severely deplete catecholamine (dopamine and norepinephrine) levels. Hypothalamus tissue was extracted on Day 6. Telencephalon tissue was extracted on Day 6. The objective of this study was to examine gene expression changes in the neuroendocrine brain in response to catecholamine depletion. A common reference design was used for both the hypothalamus and telencephalon groups: Hypothalamus: 3 independent groups of hypothalami from sexually mature female goldfish treated with 50 ug/g MPTP + 240 ug/g aMPT hybridized against a common vehicle control. An additional group was used for a dye-swap. Slides 4, 17, 21, 52. Telencephalon: 3 independent groups of telencephali from sexually mature female goldfish treated with 50 ug/g MPTP + 240 ug/g aMPT hybridized against a common vehicle control. An additional group was used for a dye-swap. Slides 28, 34, 43, 48.

2013-03-20 | E-GEOD-16044 | biostudies-arrayexpress

Secretoneurin: A new hormone? Auratus: The goldfish environmental genomics project

Project description:The objectives of this study were to examine the effect of secretoneurin (SN) on pituitary gene expression. A mixture of goldfish pituitaries from both males and females were dispersed using Typsin type II and DNase II enzymes. The cells were plated in 24-well culture plates at a density of ~2.5 x 105 cells/well. After at least 12-hr plating period, cells were washed with testing medium and pre-incubated for 1 hr to stabilize basal LH secretion. Dispersed pituitary cells were treated with 10 nM gfSN for 12 hours in static incubation after which the cells were carefully washed by 1X PBS. Total RNA was isolated by utilizing RNeasy Micro Kit (QIAGEN GmbH, Hilden, Germany) following the company’s standard protocol. RNA concentrations were determined using the NanoDrop ND-100 spectrophotometer (Thermo Scientific, Wilmington, DE). cDNA synthesis, cDNA purification and microarray slide hybridization followed our validated and established microarray protocol (Martyniuk et al., 2006; Zhang et al., 2009; Popesku et al., 2010). 4 control and 4 treatment samples were hybridized using dual channel micrarray with cy3 and cy5

2011-10-05 | E-GEOD-32629 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: dopamine antagonists

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-03-20 | E-GEOD-15763 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: fadrozole in the telencephalon

Project description:Sexually mature female goldfish were exposed (waterborne) to 50 ug/L fadrozole or untreated water (control) to examine the global gene expression of fadrozole on the telencephalon. A common reference design was used: 5 independent groups of telencephalonic tissue from sexually mature female goldfish exposed to 50 ug/L of waterborne fadrozole were hybridized against a common pool of unexposed fish. Slides 4, 14, 22, 33, 44.

2010-05-16 | E-GEOD-14558 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data