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Comparison between GT and ntcA-overexpressing strains of cyanobacteria [nitrogen-replete conditions]

ABSTRACT: NtcA regulates primary in a unicelluar cyanobacterium Synechocystis sp. PCC6803. We constructed an ntcA-overexpressing strain, named NOX10, by introducing the ntcA genes fusing psbAII promoter by homologous recombination. The transcript profiles of parental wild-type strain GT and NOX10 were compared by microarray CyanoChip (Takrara bio.). Experiments were performed three times with biologically independent RNA.

ORGANISM(S): Synechocystis sp. PCC 6803

PROVIDER: GSE54485 | GEO | 2014/07/01

SECONDARY ACCESSION(S): PRJNA236619

REPOSITORIES: GEO

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Comparison between GT and ntcA-overexpressing strains of cyanobacteria [nitrogen-replete conditions]

Project description:NtcA regulates primary in a unicelluar cyanobacterium Synechocystis sp. PCC6803. We constructed an ntcA-overexpressing strain, named NOX10, by introducing the ntcA genes fusing psbAII promoter by homologous recombination. The transcript profiles of parental wild-type strain GT and NOX10 were compared by microarray CyanoChip (Takrara bio.). Experiments were performed three times with biologically independent RNA. Total RNAs from three independent wild-type (GT) and three independent ntcA-overexpressing strains were differently labeled by Cy3 or Cy5, followed by hybridization on CyanoChip.

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Comparison between GT and ntcA-overexpressing strains of cyanobacteria

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-07-01 | GSE54488 | GEO