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Comparison between GT and hoxH mutant strains of cyanobacteria

ABSTRACT: HoxH is a subunit of hydrogenase in a unicelluar cyanobacterium Synechocystis sp. PCC6803. We constructed hoxH mutant strain, which the mRNA levels of hoxH reduced to 50% of the wild-type.

ORGANISM(S): Synechocystis sp. PCC 6803

PROVIDER: GSE76625 | GEO | 2016/06/01

SECONDARY ACCESSION(S): PRJNA308195

REPOSITORIES: GEO

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Comparison between GT and hoxH mutant strains of cyanobacteria

Project description:HoxH is a subunit of hydrogenase in a unicelluar cyanobacterium Synechocystis sp. PCC6803. We constructed hoxH mutant strain, which the mRNA levels of hoxH reduced to 50% of the wild-type. Total RNAs from three independent wilde-type (GT) and three independent hoxH mutant were labeled by Cy3 followed by hybridyzation on a microarry chip generated by Agilent Technologies (Design ID 036279). Microarray is designed by Prof. Tomohisa Hasunuma, Kobe University.

2016-06-01 | E-GEOD-76625 | biostudies-arrayexpress

Comparison between GT and ntcA-overexpressing strains of cyanobacteria [nitrogen-depleted conditions]

Project description:NtcA regulates primary in a unicelluar cyanobacterium Synechocystis sp. PCC6803. We constructed an ntcA-overexpressing strain, named NOX10, by introducing the ntcA genes fusing psbAII promoter by homologous recombination. The transcript profiles of parental wild-type strain GT and NOX10 were compared by microarray CyanoChip (Takrara bio.). Experiments were performed two times with biologically independent RNA.

2014-07-01 | GSE54487 | GEO

Comparison between GT and ntcA-overexpressing strains of cyanobacteria [nitrogen-replete conditions]

2014-07-01 | GSE54485 | GEO

Comparison between GT and rre37 overexpressed strains of cyanobacteria

Project description:Rre37 activates sugar catabolism in a unicelluar cyanobacterium Synechocystis sp. PCC6803. We constructed rre37-overexpressing strain, named ROX370, by introducing the rre37 genes fusing psbAII promoter by homologous recombination. The transcript profiles of parental wild-type strain GT and ROX370 were compared by microarray CyanoChip (Takrara bio.). Experiments were performed by three times with biologically independent RNA. The results showed that genes for the oxidaditive pentose phosphate pathway and glycogen catabolism were induced by rre37 overexpression.

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Comparison between GT and SigE overexpressed strains of cyanobacteria

Project description:SigE is a global regulator for sugar catabolism in a unicelluar cyanobacterium Synechocystis sp. PCC6803. We constructed SigE-overexpressing strain, named GOX50, by introducing the sigE genes fusing psbAII promoter by homologous recombination. The transcript profiles of parental wild-type strain GT and GOX50 were compared by microarray CyanoChip (Takrara bio.). Experiments were performed by three times with biologically independent RNA. The results showed that genes for the oxidaditive pentose phosphate pathway and glycogen catabolism were induced by SigE overexpression.

2011-07-09 | GSE27406 | GEO

Comparison between GT and ntcA-overexpressing strains of cyanobacteria [nitrogen-depleted conditions]

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2014-07-01 | E-GEOD-54485 | biostudies-arrayexpress

Comparison between GT and rre37 overexpressed strains of cyanobacteria

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2011-07-09 | E-GEOD-27406 | biostudies-arrayexpress

Comparison of wild-type Synechocystis sp. PCC 6803 with strains overexpressing wild-type or 5’-sensing-deficient RNase E

Project description:We compared transcriptomic changes, 5'-triphosphorylated (TSS) and 5'-monophosphorylated (PSS) RNA ends of different strains of the cyanobacterium Synechocystis sp. PCC6803. Comparison encompassed wild-type Synechocystis (WT), a strain overexpressing RNase E and RNase HII (rne(WT)) and a strain overexpressing 5’-sensing-deficient RNase E and RNase HII (rne(5p)). Analysis of changing 5'-monophosphorylated ends revealed 5’ sensing depedent processing sites on a transcriptome-wide level.

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