Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression in wild-type and transgenic plants overexpressing rice topoisomerase6 genes

ABSTRACT: 10-day-old wild-type and homozygous transgenic Arabidopsis seedlings (overexpressing OsTOP6A3 and OsTOP6B) grown under normal growth conditions were used for total RNA isolation. The 5 micrograms of each total RNA sample was processed for microarray analysis according to Affymetrix protocol. Keywords: Arabidopsis, wild-type and transgenic seedlings, differential gene expression

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE5465 | GEO | 2006/08/09

SECONDARY ACCESSION(S): PRJNA95977

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression in wild-type and transgenic plants overexpressing rice OsTOP6A1 gene

2007-12-26 | GSE6812 | GEO

Gene expression in wild-type and transgenic plants overexpressing rice OsTOP6A1 gene

Project description:10-day-old wild-type and homozygous transgenic Arabidopsis seedlings (overexpressing rice topoisomerase 6 subunit A1; OsTOP6A1) grown under normal growth conditions were used for total RNA isolation. The 5 micrograms of each total RNA sample was processed for microarray analysis according to Affymetrix protocol. Experiment Overall Design: The experiment involves the study of differential gene expression in transgenic Arabidopsis seedlings overexpressing rice topoisomerase 6 subunit A1 (OsTOP6A1) cDNA as compared to wild-type.Three biological replicates for each sample were used for microarray analysis.

2008-06-15 | E-GEOD-6812 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type and transgenic plants overexpressing rice topoisomerase6 genes

Project description:10-day-old wild-type and homozygous transgenic Arabidopsis seedlings (overexpressing OsTOP6A3 and OsTOP6B) grown under normal growth conditions were used for total RNA isolation. The 5 micrograms of each total RNA sample was processed for microarray analysis according to Affymetrix protocol. Experiment Overall Design: The experiment involves the study of differential gene expression in transgenic Arabidopsis seedlings overexpressing rice topoisomerase 6 subunit A (OsTOP6A3) and subunit B (OsTOP6B) cDNAs as compared to wild-type.Two biological replicates for each sample were used for microarray analysis.

2008-06-13 | E-GEOD-5465 | biostudies-arrayexpress

Arabidopsis thaliana: transgenic plants vs wild type

Project description:Arabidopsis thaliana: transgenic plants vs wild type

| PRJNA1055021 | ENA

Arabidopsis thaliana: transgenic plants vs wild type

Project description:Transcriptional profiling of Arabidopsis thaliana 12-days old seedlings comparing Col-0 wild type with transgenic plants with altered expression of dual-targetting plastid/mitochondrial organellar RNA-polymerase RPOTmp. Transgenic plants used for experiment were: overexpressor plants obtained by transformation of Col-0 WT plants with genetic constructs created in [Tarasenko et al., 2016] contained catalytic part of RPOTmp enzyme with transit peptides of RPOTm (mitochondrial) and RPOTp (plastid) by agrobacterial transformation; plants with complementation of RPOTmp functions in mitochondria or chloroplasts obtained from transformation of GABI_286E07 rpotmp knockout-mutant plants with genetic constructs created in [Tarasenko et al., 2016]. Goal was to determine the effects of RPOTmp knockout/overexpression on global Arabidopsis thaliana gene expression.

2023-12-25 | GSE251661 | GEO

Differentially expressed genes between WT and AtC3H17-overexpressing transgenic plants

Project description:We characterized the biological and molecular functions of AtC3H17, a unique Arabidopsis gene encoding a non-tandem CCCH zinc finger protein, in plant development. To investigate the downstream regulatory mechanisms of AtC3H17, whole genome microarray expression profiling was carried out. The experiment was designed to identify differentially expressed genes between WT and AtC3H17-overexpressing transgenic plants (AtC3H17 OXs) grown for 14 days under SD growth conditions. Interestingly, the most up-regulated genes were 12S seed storage globulin genes, CRUCIFERIN A (CRA1) and CRUCIFERIN 3 (CRU3), and seed oil-body protein genes such as OLEOSIN 1 (OLEO1) and OLEO2 in AtC3H17 OXs compared with WT. We performed quantitative RT-PCR and confirmed that transcription levels of CRU3, OLEO1, OLEO2, and 2S seed storage albumin 1 (At2S1) were higher in AtC3H17 OX seedlings than in WT seedlings.

2015-12-05 | GSE75702 | GEO

Transcription profiling of Arabidopsis wild-type and transgenic plants overexpressing the AtCPK1 gene

Project description:Transcription profiles of wild-type and transgenic Arabidopsis plants overexpressing the AtCPK1 gene

2011-01-07 | E-MEXP-2532 | biostudies-arrayexpress

Expression analysis of RCc3:OsNAC5 and GOS2:OsNAC5 transgenic rice plants

Project description:For identification of genes up-regulated in RCc3:OsNAC5, GOS2:OsNAC5 plants, total RNA (100 μg) was prepared from root tissues of 14-d-old transgenic and non-transgenic rice seedlings (Oryza sativa cv Nipponbare) grown under normal growth conditions.

2012-03-01 | GSE31856 | GEO

Expression analysis of RCc3:OsNAC9 and GOS2:OsNAC9 transgenic rice plants

Project description:For identification of genes up-regulated in RCc3:OsNAC1, GOS2:OsNAC1 plants, total RNA (100 μg) was prepared from root tissues of 14-d-old transgenic and non-transgenic rice seedlings (Oryza sativa cv Nipponbare) grown under normal growth conditions.

2012-03-01 | GSE31855 | GEO

Expression analysis of RCc3:OsNAC6 and GOS2:OsNAC6 transgenic rice plants

Project description:For identification of genes up-regulated in RCc3:OsNAC6, GOS2:OsNAC6 plants, total RNA (100 μg) was prepared from root tissues of 14-d-old transgenic and non-transgenic rice seedlings (Oryza sativa cv Nipponbare) grown under normal growth conditions.

2012-03-01 | GSE31857 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data