3C-seq of HT29 cells with CCAT1-L knockdown or scramble knockdown

ABSTRACT: CCAT1-L is a highly expressed long noncoding RNA located in the colorectal cancer specific super enhance region about 500 kb upstream of MYC gene. Knockdown of CCAT1-L significantly down-regulated interaction frequency between CCAT1 and MYC locus and repress MYC expression, suggesting a long-range chromatin interaction between CCAT1-L and MYC locus maintained by CCAT1-L underlie the MYC regulation. To further validate this hypothesis, multiplexed 3C sequencing (3C-seq) was employed to evaluate chromatin interaction strength between CCAT1-L and MYC locus in CCAT1-L knockdown and scramble knockdown (Scr) HT29 cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE55261 | GEO | 2014/02/22

SECONDARY ACCESSION(S): PRJNA239020

REPOSITORIES: GEO