Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq study of major P.aeruginosa PA14 transcription factors

ABSTRACT: Gene regulation via transcription factors influences the metabolic, adaptive and pathogenic capabilities of the organism. We report the transcriptomes of the mutants of six major P. aeruginosa PA14 trancription factors - RhlR, LasR, Anr, GacA, FleQ and CbrB.

ORGANISM(S): Pseudomonas aeruginosa

PROVIDER: GSE55328 | GEO | 2016/03/01

SECONDARY ACCESSION(S): PRJNA239334

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA-seq study of major P.aeruginosa PA14 transcription factors

Project description:Gene regulation via transcription factors influences the metabolic, adaptive and pathogenic capabilities of the organism. We report the transcriptomes of the mutants of six major P. aeruginosa PA14 trancription factors - RhlR, LasR, Anr, GacA, FleQ and CbrB. The P. aeruginosa PA14 transposon mutants were analyzed by RNA-seq. All samples were cultivated in LB medium until reaching an OD600 of 2.0. For each biological replicate, three cultures were pooled for RNA extraction, library preparation and sequencing.

2016-03-01 | E-GEOD-55328 | biostudies-arrayexpress

Expression profile for PA14 lasR rhlR double mutant with H2O2 stress

Project description:Quorum sensing controls the expression of multiple virulence factors. PA14 genes lasR and rhlR are necessary for quorum sensing via homoserine lactones. A PA14 lasR rhlR deficient mutant exhibits a reduced oxidative stress response. Here we conducted a microarray to determine oxidative stress response gene regulation mediated by the homoserine lactone quorum sensing circuits. A PA14 lasR rhlR deficient mutant was compared to the wild-type with and without H2O2 stress.

2015-01-09 | E-GEOD-54245 | biostudies-arrayexpress

Expression profile for PA14 lasR rhlR double mutant with H2O2 stress

2015-01-09 | GSE54245 | GEO

Expression data from Arabidopsis seedlings treated with Pseudomonas aeruginosa (strain PA14)

Project description:Early stages of host microbe adaptations involve 'system status changes' (rewiring of pre-existing cellular signaling networks and components) of the host and microbe. We posited that under certain environmental conditions these changes leads to maladaptations and favor emergence of new infectious diseases, and these adaptations will have characteristic signatures representative of the adaptation. Here using Arabidopsis seedlings in a submerged environment treated with P. aerugionsa, we show one such rewired regulation where the master two-component regulator GacA (previously shown to act upstream of quorum sensing, including the regulator LasR, that in turn controls a subset of virulence factors) is completely dispensable. The gacA mutant behaves similar to wild type P. aeruginosa (strain PA14) by a number of read-outs. Consistent with that, the gene expression data here indicates that the transcriptome pattern of the host is identical when treated with wild type PA14 or PA14-gacA mutant.

2011-01-22 | GSE26084 | GEO

Expression data from Arabidopsis seedlings treated with Pseudomonas aeruginosa (strain PA14)

2011-01-22 | E-GEOD-26084 | biostudies-arrayexpress

Transcription profiling of P.aeruginosa PA14 and P.aeruginosa PA14 metR::phoA

Project description:Five biological repeats of P.aeruginosa PA14 and five biological repeats of P.aeruginosa PA14 metR::phoA were grown in liquid BM2 swarming media containing 62mM potassium phosphate buffer [pH7], 2 mM MgSO4, 10uM FeSO4, 0.4% (wt/vol) glucose, and 0.1% (wt/vol) casamino acids. Microarray experiments were done using microarray slides and protocols from TIGR on samples taken from mid-log phase (OD600 0.4-0.5)cells.

2009-08-20 | E-FPMI-19 | biostudies-arrayexpress

Transcription profiling of P.aeruginosa PA14 and P.aeruginosa PA14 metR::phoA grown on swarming plates

Project description:Four biological repeats of P.aeruginosa PA14 and four biological repeats of P.aeruginosa PA14 metR::phoA were grown in BM2 swarming media plates containing 62mM potassium phosphate buffer [pH7], 2 mM MgSO4, 10uM FeSO4, 0.4% (wt/vol) glucose, 0.1% (wt/vol) casamino acids and 0.5% agar. Microarray experiments were done using microarray slides and protocols from TIGR on samples harvested from the dendritic swarm colony growth, 2 to 3 mm of the swarm zone edge.

2009-08-20 | E-FPMI-20 | biostudies-arrayexpress

C. elegans gene expression in response to the pathogenic P. aeruginosa strain PA14.

Project description:Analysis of differential gene expression in C. elegans adults exposed to three different bacteria: E. coli strain OP50, wild-type P. aeruginosa PA14 and gacA mutant PA14. Samples were analyzed at 4 hours and 8 hours after exposure to the different bacteria. These studies identified C. elegans genes induced by pathogen infection. Keywords: Time course, response to pathogen infection

2007-02-08 | GSE5793 | GEO

Transcription profiling of C. elegans gene response to the pathogenic P. aeruginosa strain PA14.

Project description:Analysis of differential gene expression in C. elegans adults exposed to three different bacteria: E. coli strain OP50, wild-type P. aeruginosa PA14 and gacA mutant PA14. Samples were analyzed at 4 hours and 8 hours after exposure to the different bacteria. These studies identified C. elegans genes induced by pathogen infection. Experiment Overall Design: Three independent biological replicates were isolated for each treatment. All treatments were performed in parallel.

2008-06-14 | E-GEOD-5793 | biostudies-arrayexpress

Microarray for suspension cells of PA14 WT with and without NE (50 uM and 500 uM) for 7 h

Project description:It has been proposed that the gastro-intestinal tract environment containing high levels of neuroendocrine hormones is important for gut-derived P. aeruginosa infections. In this study, we report that the hormone norepinephrine increases P. aeruginosa PA14 growth, virulence factor production, invasion of HCT-8 epithelial cells, and swimming motility in a concentration-dependent manner. Transcriptome analysis of P. aeruginosa exposed to 500 µM, but not 50 µM, norepinephrine for 7 h showed that genes involved in the regulation of the virulence determinants pyocyanin, elastase, and Pseudomonas quinolone signal (PQS, 2-heptyl-3-hydroxy-4-quinolone) were up-regulated. The production of rhamnolipids, which are also important in P. aeruginosa infections, was significantly decreased on semi-solid surfaces, but not in planktonic cultures, upon exposure to norepinephrine. Swarming motility, a phenotype that is directly influenced by rhamnolipids, was also decreased upon 500 µM norepinephrine exposure. The increase in the transcriptional activation of lasR, but not that of rhlR, suggest that the effects of norepinephrine are mediated primarily through the las quorum sensing pathway. Together, our data strongly suggests that norepinephrine can play an important role in gut-derived infections by increasing the pathogenicity of P. aeruginosa PA14.

2009-08-27 | GSE13326 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data