Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analysis of global gene expression in pluripotent stem cell (PSC) derived neural stem cells (NSCs) and neurons and astrocytes differentiated from these NSCs

ABSTRACT: Gene expression was compared between two control PSC lines and neurons and astrocytes differentiated from each line.

ORGANISM(S): Homo sapiens

PROVIDER: GSE55379 | GEO | 2014/02/27

SECONDARY ACCESSION(S): PRJNA239436

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Analysis of global gene expression in pluripotent stem cell (PSC) derived neural stem cells (NSCs) and neurons and astrocytes differentiated from these NSCs

Project description:Gene expression was compared between two control PSC lines and neurons and astrocytes differentiated from each line. Total RNA obtained from fetal astrocytes from two sources compared to total RNA obtained from two neuronal stem cell lines.

2014-02-27 | E-GEOD-55379 | biostudies-arrayexpress

Direct reprogramming of human astrocytes into neural stem cells and neurons

Project description:Ectopic expression of the reprogramming factors OCT4, SOX2, or NANOG into human astrocytes in specific cytokine/culture conditions activated the neural stem gene program and induced generation of cells expressing neural stem/precursor markers. Here we compare the whole gene expression profile of primary human astrocytes (Astro) with neural stem cells (HNSC) derived from astrocytes reprogramming

2013-12-01 | E-GEOD-36145 | biostudies-arrayexpress

Direct reprogramming of human astrocytes into neural stem cells and neurons (MeDIP)

Project description:Ectopic expression of the reprogramming factors OCT4, SOX2, or NANOG into human astrocytes in specific cytokine/culture conditions activated the neural stem gene program and induced generation of cells expressing neural stem/precursor markers (ASTRO-NSC). To evaluate the epigenetic changes associated with this reprogramming, we analyzed the DNA methylation patterns of Astro-NSC relative to untransfected astrocytes. We compared three human AstroNANOG-NSC clones to the astrocytes from which they were derived using NimbleGen 3x720K CpG Island Plus RefSeq Promoter Arrays

2013-01-05 | E-GEOD-42908 | biostudies-arrayexpress

ChIP-seq experiment of PHF8 in Astrocytes differentiated from mouse NSCs

Project description:In this study we analyzed the contribution of PHF8 histone demethylase to astrocytes differentiation from mouse neural stem cells. We found that PHF8 depletion affects astocytes differentiation. Moreover, PHF8 is crucial for synaptogenesis in neurons/astrocytes cocultures. Genome wide analysis demonstrated that PHF8 controls the expression of critical astrogenic and synaptogenic genes by keeping low levels of H4K20me1 at promoters. PHF8 depletion induces aberrant astrocytes phenotype and caused a significant decrease in miniature excitatory postsynaptic currents (mEPSC) frequency and amplitude in neurons/astrocytes coclutures. These data reveal a new role of PHF8 in astrocyte differentiation and function, modulating neuronal synapse. Thus, lack of histone demethylase activity associated to PHF8 mutations might led to synapse disfunction that could directly impact into X-linked intellectual disabilities.

2021-06-14 | GSE141969 | GEO

Single-cell sequencing of human induced pluripotent stem cell-derived ventral midbrain astrocytes

Project description:Regional specificity of stem cell-derived astrocytes is believed to be an important prerequisite for their applications in disease modelling and cell-based therapies. The regional identity of these astrocytes is often defined by the positional characteristics of their antecedent, stem cell-derived neural progenitors patterned to a fate of interest, with the assumption that the positional specification is to be preserved by the derived astrocytes. Using a human iPSC line designed for tracing midbrain floor plate derivatives, here we show that lineage composition of the derived astrocytes is not a faithful recapitulation of the founder progenitor population, as demonstrated by the loss of floor plate differentiated progeny in the final astrocyte products. Using deep single cell RNA sequencing, we identified distinct transcriptomic signatures of midbrain floor plate-derived astrocytes. Our study highlights the need for rigorous characterisation of PSC-derived regional astrocytes and provides a valuable resource for assessing midbrain floor plate-derived human astrocytes.

2024-09-24 | GSE252624 | GEO

Astrocytes from mouse NSCs

Project description:Astrocytes from mouse NSCs

| PRJNA595476 | ENA

Analysis of global gene expression in fetal astrocytes and pluripotent stem cell derived neural stem cells

Project description:Gene expression was compared between two sets of commercially obtained fetal astrocytes and two sets of neuronal stem cell lines derived from iPSC and ES cells. Pathways and genes critical for astrocyte development and maintenance were identified in the analysis. Total RNA obtained from fetal astrocytes from two sources compared to total RNA obtained from two neuronal stem cell lines.

2014-02-02 | E-GEOD-53404 | biostudies-arrayexpress

Direct reprogramming of human astrocytes into neural stem cells and neurons (MeDIP)

Project description:Ectopic expression of the reprogramming factors OCT4, SOX2, or NANOG into human astrocytes in specific cytokine/culture conditions activated the neural stem gene program and induced generation of cells expressing neural stem/precursor markers (ASTRO-NSC). To evaluate the epigenetic changes associated with this reprogramming, we analyzed the DNA methylation patterns of Astro-NSC relative to untransfected astrocytes.

2013-01-05 | GSE42908 | GEO

Transcription profiling of human neural progenitors, neurons, and regionally specified astrocytes from human embryonic stem cells

Project description:These arrays were conducted in order to confirm the generation of astrocytes from human embryonic stem cells and to determine expression differences between astrocyte subtypes. Experiment Overall Design: Human embryonic stem cells were differentated in vitro to neuroepithelial cells (day-17), neurons (day-50), or astrocytes (day-180) after specification with the morphogens retinoic acid (RA) or FGF8 from days 10-21.

2009-11-28 | E-GEOD-18959 | biostudies-arrayexpress

Direct reprogramming of human astrocytes into neural stem cells and neurons

2013-12-01 | GSE36145 | GEO