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Streptococcus mutans UA159 grown in the presence of glucose or maltose pH7 and pH5

ABSTRACT: Transcriptional analysis of the effects of sugar source in S. mutans

ORGANISM(S): Streptococcus mutans UA159 Streptococcus mutans

PROVIDER: GSE55952 | GEO | 2014/06/01

SECONDARY ACCESSION(S): PRJNA241424

REPOSITORIES: GEO

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Streptococcus mutans UA159 grown in the presence of glucose or maltose pH7 and pH5

Project description:Transcriptional analysis of the effects of sugar source in S. mutans RNA was extracted from 4 replicate samples of S. mutans UA159, UA159 grown in glucose or maltose in continuous culture to steady-state pH values of 7 or 5. RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

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Project description:Transcriptional analysis of the regulator FabT in S. mutans RNA was extracted from 4 replicate samples of S. mutans UA159 and MU1591 (M-bM-^HM-^FfabT) grown in continuous culture to steady-state pH values of 7 and 5. RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

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Streptococcus mutans UA159 vs. fabM mutant strain (UR117), pH7 vs. pH5/5.5

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Project description:Transcriptional analysis of glucose shock vs. steady-state growth in the parent strain and an acid sensitive mutant strain of S. mutans RNA was extracted from 4 replicate samples of S. mutans UA159 and UR117 (fabM mutant strain) grown in continuous culture to a steady-state pH value of 7. The cultures were exposed to a glucose shock (200mM) and samples were collected upon achieving culture pH value of 5.5. pH control was re-established and cultures were allowed to grow to a steady-state pH value of 5 (for UA159) and 5.5 (for fabM mutant). RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

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Streptococcus mutans UA159 with or without 8.4% oxygen vs. nox mutant strain. pH7

Project description:Transcriptional analysis of the effects of oxygen concentraion in S. mutans

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Streptococcus mutans UA159 with or without 8.4% oxygen vs. nox mutant strain. pH5

Project description:Transcriptional analysis of the effects of oxygen concentraion in S. mutans

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Streptococcus mutans UA159 with or without 8.4% oxygen vs. nox mutant strain. pH5

Project description:Transcriptional analysis of the effects of oxygen concentraion in S. mutans RNA was extracted from 3 or 4 replicate samples of S. mutans UA159, UA159 grown in 8.4 % oxygen concentration and MU1020 (M-bM-^HM-^Fnox) grown in continuous culture to steady-state pH values of 5. RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

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Project description:Transcriptional analysis of the effects of oxygen concentraion in S. mutans RNA was extracted from 3 or 4 replicate samples of S. mutans UA159, UA159 grown in 8.4 % oxygen concentration and MU1020 (M-bM-^HM-^Fnox) grown in continuous culture to steady-state pH values of 7. RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

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