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Single cell gene expression of early E8.5 pioneer neural crest cells and paraxial mesoderm

ABSTRACT: Laser capture microdissection (LCM) was used to isolate cells from the principal critical micro-regions, whose development, differentiation and signaling interactions are responsible for the construction of the mammalian face. At E8.5, as migrating neural crest cells begin to exit the neural fold/epidermal ectoderm boundary, we examined the facial mesenchyme, composed of neural crest and paraxial mesoderm cells, as well as cells from adjacent neuroepithelium. For these 11 samples, we used a single cell isolation and cDNA generation protocol followed by a standard Affymetrix labeling protocol to better define the gene expression states of the early E8.5 pioneer neural crest and paraxial mesoderm cells. Using a combination of statistical and expression level filtering criteria, we have identified expression patterns present within these embryonic cell populations. The genes identified through this study are highly enriched for known critical genes as well as many genes not previously known to be cell type specific at this stage.

ORGANISM(S): Mus musculus

PROVIDER: GSE55964 | GEO | 2014/03/18

SECONDARY ACCESSION(S): PRJNA241447

REPOSITORIES: GEO

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Single cell gene expression of early E8.5 pioneer neural crest cells and paraxial mesoderm

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