Transcriptomics

Dataset Information

0

Changes in Gene Expression in Brassica oleracea Shoots during Phosphate Starvation (Cross-species study)


ABSTRACT: The aim of this study is to study gene expression in Brassica oleracea in shoot tissues of plants grown under contrasting P supplies (see Hammond JP et al., 2003, Plant Physiology, 132, 578-596 for background). Seeds of B. oleracea (var. alboglabra, A12dH) were first washed in 70% (v/v) ethanol/water, rinsed in distilled water and surface sterilised using 50% (v/v) domestic bleach/water. Seeds were rinsed and imbibed for 3 to 5 days in sterile distilled water at 4°C to break dormancy. Following imbibition, B. oleracea seeds were sown in un-vented, polycarbonate culture boxes (Sigma-Aldrich Company Ltd., Dorset UK). Seedlings were grown for 21 days on perforated polycarbonate discs (diameter 91 mm by 5 mm) placed on 75 ml of 0.8% (w/v) agar containing 1% (w/v) sucrose and a basal salt mix. Roots grew into the agar, but shoots remained on the opposite side of the disc. After 21 days, seedlings were transferred, still on polycarbonate discs, to a hydroponics system situated in a Saxcil growth cabinet (16 h daylength, set to 22°C and 80% humidity). Each polycarbonate disc was placed on a light-proof 500 ml beaker over 450 ml of nutrient solution. After 7 days, half the plants were transferred to nutrient solution containing no phosphate and the other half remained on full nutrient solution (control plants). Shoots were harvested 100 h after the withdrawal of P. !Samples were snap frozen in liquid nitrogen. RNA was extracted using the TRIzol extraction method and cleaned through a Qiagen RNeasy column. Experimenter name = Martin Broadley Experimenter phone = 0115 951 6382 Experimenter fax = 0115 951 6334 Experimenter institute = University of Nottingham Experimenter address = Plant Sciences Division Experimenter address = School of Biosciences Experimenter address = University of Nottingham Experimenter address = Sutton Bonington Experimenter address = Loughborough Experimenter zip/postal_code = LE12 5RD Experimenter country = UK Keywords: growth_condition_design

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE5614 | GEO | 2007/01/08

SECONDARY ACCESSION(S): PRJNA96907

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2008-06-13 | E-GEOD-5614 | biostudies-arrayexpress
2007-01-08 | GSE5736 | GEO
2008-06-14 | E-GEOD-5736 | biostudies-arrayexpress
2007-01-22 | GSE6825 | GEO
2007-01-08 | GSE5611 | GEO
2008-06-13 | E-GEOD-5611 | biostudies-arrayexpress
2015-05-26 | PXD001567 | Pride
2007-01-08 | GSE5744 | GEO
2008-06-15 | E-GEOD-6825 | biostudies-arrayexpress
2007-09-01 | GSE8246 | GEO