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Gene expression profiling from pooled samples of subcutaneous adipose tissue of NAPE-WT or NAPE-KO mice fed either with a control diet or a high-fat diet.

ABSTRACT: Mice knocked-out or wild type for the NAPE PLD gene specifically in adipose tissue, were recruited for this expression profiling experiment. Each group of mice (WT versus cKO) were fed with a control diet or a high fat diet. Then mice were sacrificed and adipose tissue samples form the subcutaneous adipose tissue were processed for RNA extraction. Total RNA of each sample was then pooled with those of the same group and treatment for microarray hybridization.

ORGANISM(S): Mus musculus

PROVIDER: GSE56852 | GEO | 2015/03/06

SECONDARY ACCESSION(S): PRJNA244767

REPOSITORIES: GEO

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Gene expression profiling from pooled samples of subcutaneous adipose tissue of NAPE-WT or NAPE-KO mice fed either with a control diet or a high-fat diet.

Project description:Mice knocked-out or wild type for the NAPE PLD gene specifically in adipose tissue, were recruited for this expression profiling experiment. Each group of mice (WT versus cKO) were fed with a control diet or a high fat diet. Then mice were sacrificed and adipose tissue samples form the subcutaneous adipose tissue were processed for RNA extraction. Total RNA of each sample was then pooled with those of the same group and treatment for microarray hybridization. two groups of mice: (I) NAPE PLD WT and NAPE PLD KO. Each group was submitted to a diet treatment: (I) control diet and (II) high fat diet.

2015-03-06 | E-GEOD-56852 | biostudies-arrayexpress

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Gene expression profiling from pooled samples of liver tissue of liver MyD88 WT mice and MyD88 liver specific KO mice fed either with a control diet or a high-fat diet.

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The effect of semaglutide on high-fat diet-induced liver cancer in obese mice

Project description:This study aims to investigate the impact of semaglutide on the expression of liver cancer proteins in obese mice induced by a high-fat diet. Sixteen obese mice were randomly divided into two groups: the high-fat diet group and the semaglutide group, each consisting of eight mice. Additionally, eight normal male mice were included as the control group. Serum samples were collected, and a differential expression analysis of total proteins in adipose tissue was performed using quantitative tandem mass spectrometry (TMT) in combination with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Significant differential proteins were identified and subjected to bioinformatics analysis. The findings revealed that these differential proteins, namely ITGAV (integrin αV), LAMC1 (laminin γ1), FABP5 (fatty acid-binding protein 5), and LPL (lipoprotein lipase), regulate the occurrence and development of liver cancer by participating in the extracellular matrix (ECM) signaling pathway and the peroxisome proliferator-activated receptor (PPAR) signaling pathway. Notably, semaglutide can decelerate the progression of liver cancer by inducing the expression of ITGAV, LAMC1, FABP5, and LPL in the adipose tissue of obese mice.

2023-12-04 | PXD047492 |

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