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Expression data from HEK wild-type cells treated with geneticin (16uM) or left untreated

ABSTRACT: In order to study the cellular response to aminoglycoside-induced mistranslation, we used a whole-genome microarray analysis of geneticin-treated versus untreated cells, which revealed a broad transcriptional response totaling 705 genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE57198 | GEO | 2014/05/01

SECONDARY ACCESSION(S): PRJNA245892

REPOSITORIES: GEO

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Expression data from HEK wild-type cells treated with geneticin (16uM) or left untreated

Project description:In order to study the cellular response to aminoglycoside-induced mistranslation, we used a whole-genome microarray analysis of geneticin-treated versus untreated cells, which revealed a broad transcriptional response totaling 705 genes. comparison of four geneticin treated biological replicates with four untreated biological replicates (selected for a fold change > 1.2, Benjamini-Hochberg corrected p-value < 0.05)

2014-05-01 | E-GEOD-57198 | biostudies-arrayexpress

HEK293 cells LC-MSMS - XBP1 mitigates aminoglycoside-induced endoplasmic reticulum stress and neuronal cell death

Project description:The iTRAQ 4-plex experiment was carried out with 2 biological replicates of untreated HEK cells (114 and 116 label) and 2 biological replicates of HEK cells treated with geneticin (16 µM) for 32 h (115 and 117 label).

2015-08-10 | PXD000933 | Pride

Identifying the transcriptomic effects of tRNA-induced proline-to-serine mistranslation in Drosophila melanogaster

Project description:Mistranslation describes the misincorporation of an amino acid into a nascent polypeptide. Mistranslation has diverse effects on multicellular eukaryotes and is implicated in several human diseases. We introduced a mistranslating serine transfer RNA (tRNA) into Drosophila melanogaster that misincorporates serine at proline codons and found that it affected male and female flies differently. Here, we compare the transcriptomic response of male and female flies to mistranslation to identify cellular pathways that underlie this sex-specific response. Both males and females downregulated genes associated with metabolism in response to proline-to-serine mistranslation. Only males downregulated genes associated with developmental processes and response to negative stimuli such as infection, whereas only females downregulated aerobic respiration and ATP synthesis genes. Both sexes upregulated genes associated with gametogenesis but females upregulated cell cycle and DNA maintenance genes, suggesting that mistranslation may compromise genome integrity in females. This transcriptomic analysis advances our understanding of how males and females respond to mistranslation and has important implications for future studies that examine the influence of mistranslation on disease.

2024-05-11 | GSE256332 | GEO

Translation Error Clusters Induced by Aminoglycoside Antibiotics

Project description:Aminoglycoside antibiotics target the ribosome and induce mistranslation, yet which translation errors induce bacterial cell death is unclear. The analysis of cellular proteins by quantitative mass spectrometry shows that bactericidal aminoglycosides induce not only single errors, but also clusters of errors in full-length proteins in vivo with as much as four amino acid substitutions in a row. The downstream errorsin a cluster are up to 10,000-fold more frequent than the first error and independent of the intracellular aminoglycoside concentration. The prevalence, length, and composition of error clusters dependsnot only on the misreading propensity of a given aminoglycoside, but also on its ability to inhibit ribosome translocation along the mRNA. Error clusters constitute a new class of misreading events in vivo that may provide the predominant source of proteotoxic stress at low aminoglycoside concentration, which is particularly important for the autocatalytic uptake of the drugs.

2021-04-06 | PXD022098 | Pride

Transcriptional response to aminoglycoside antibiotics in cochlear hair cells at 3 hour

Project description:We purified Atoh1-GFP positive hair cells from organotypic cultures of P1 cochlea 3 hours after 0.5mM gentamicin treatment and performed RNA sequencing to profile the early transcriptional response of hair cells to aminoglycoside antibiotics. Levels of mRNA in gentamicin-treated hair cells (three replicates) were compared to untreated hair cells (three replicates). GFP negative, non-hair cells populations from treated organs were compared to those from untreated organs (three replicates for each condition).

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Proteome of BaF3-mEpoR cells stimulated with 5 U/ml Epo, 10 ng/ml IL-3 or left untreated

Project description:The proteome of BaF3-mEpoR cells stimulated with 5 U/ml Epo, 10 ng/ml IL-3 or left untreated were analyzed by label free LC-MS/MS.

2022-09-28 | PXD035181 | Pride