Transcriptomics

Dataset Information

0

Identifying the transcriptomic effects of tRNA-induced proline-to-serine mistranslation in Drosophila melanogaster


ABSTRACT: Mistranslation describes the misincorporation of an amino acid into a nascent polypeptide. Mistranslation has diverse effects on multicellular eukaryotes and is implicated in several human diseases. We introduced a mistranslating serine transfer RNA (tRNA) into Drosophila melanogaster that misincorporates serine at proline codons and found that it affected male and female flies differently. Here, we compare the transcriptomic response of male and female flies to mistranslation to identify cellular pathways that underlie this sex-specific response. Both males and females downregulated genes associated with metabolism in response to proline-to-serine mistranslation. Only males downregulated genes associated with developmental processes and response to negative stimuli such as infection, whereas only females downregulated aerobic respiration and ATP synthesis genes. Both sexes upregulated genes associated with gametogenesis but females upregulated cell cycle and DNA maintenance genes, suggesting that mistranslation may compromise genome integrity in females. This transcriptomic analysis advances our understanding of how males and females respond to mistranslation and has important implications for future studies that examine the influence of mistranslation on disease.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE256332 | GEO | 2024/05/11

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2015-11-06 | GSE65718 | GEO
2021-09-18 | PXD028498 | Pride
2022-08-12 | PXD032063 | Pride
2021-05-17 | PXD025934 | Pride
2015-11-06 | E-GEOD-65718 | biostudies-arrayexpress
2021-05-11 | GSE174145 | GEO
2016-10-27 | E-SYBR-13 | biostudies-arrayexpress
2019-09-24 | GSE115153 | GEO
2022-02-22 | PXD026636 | Pride
2021-11-03 | PXD028496 | Pride