Route specific dermal responses to vaccinia virus infection
Ontology highlight
ABSTRACT:
ORGANISM(S): Oryctolagus cuniculus
PROVIDER: GSE57564 | GEO | 2014/05/13
SECONDARY ACCESSION(S): PRJNA246710
REPOSITORIES: GEO
Similar Datasets
Project description:Transcriptional profiles of rabbit skin infected via the intradermal or scarification route of infeciton assessing the early time post infection. Transcriptional profiles were assessed at 4hrs, 24hrs, and 48hrs post infection for each route of infection. Transcript variation is as compared to normal, uninfected rabbit skin. Two condition, three time points per condition. 3 biological replicates per time point per condition. Normal rabbit skin was used as the control.
2014-05-13 | E-GEOD-57564 | biostudies-arrayexpress
Project description:Vaccination is one of the most efficient public healthcare measures to fight infectious diseases. Nevertheless, the immune mechanisms induced in vivo by vaccination are still unclear. The route of administration, an important vaccination parameter, can substantially modify the quality of the response. How the route of administration affects the generation and profile of immune responses is of major interest. Here, we aimed to extensively characterize the profiles of the innate and adaptive response to vaccination induced after intradermal, subcutaneous, or intramuscular administration with a modified vaccinia virus Ankara model vaccine in non-human primates. The adaptive response following subcutaneous immunization was clearly different from that following intradermal or intramuscular immunization. The subcutaneous route induced a higher level of neutralizing antibodies than the intradermal and intramuscular vaccination routes. In contrast, polyfunctional CD8+ T-cell responses were preferentially induced after intradermal or intramuscular injection. We observed the same dichotomy when analyzing the early molecular and cellular immune events, highlighting the recruitment of cell populations, such as CD8+ T lymphocytes and myeloid-derived suppressive cells, and the activation of key immunomodulatory gene pathways. These results demonstrate that the quality of the vaccine response induced by an attenuated vaccine is shaped by early and subtle modifications of the innate immune response. In this immunization context, the route of administration must be tailored to the desired type of protective immune response. This will be achieved through systems vaccinology and mathematical modeling, which will be critical for predicting the efficacy of the vaccination route for personalized medicine.
2021-05-17 | E-MTAB-10309 | biostudies-arrayexpress
Project description:The study aimed at defining the skin innate responses after intradermal injection of flagellin. For this purpose, pigs were immunized by intradermal route with flagellin and H1N1 antigen. Skin was then sampled for microarray analyses.
2016-01-31 | GSE72256 | GEO
Project description:The study aimed at defining the local and systemic innate responses after intradermal injection of flagellin. For this purpose, mice were immunized by intradermal route with flagellin. Skin and blood were then sampled at selected times for microarray analyses. Mice were then immunized with the TLR5 agonist flagellin by intradermal route. Blood samples and punch biopsies of skin were sampled post-immunization and on mock animals. Total RNA was extracted and microarray experiments were performed as single-color hybridizations on Agilent 4x44K mouse whole genome catalog arrays (Agilent-014868) according supplier's recommendations.
2016-07-03 | E-GEOD-72016 | biostudies-arrayexpress
Project description:The study aimed at defining the local and systemic innate responses after intradermal injection of flagellin. For this purpose, mice were immunized by intradermal route with flagellin. Skin and blood were then sampled at selected times for microarray analyses.
2016-01-01 | GSE72016 | GEO
Project description:Modified Vaccinia Ankara (MVA) was recently approved as a Smallpox vaccine. Transmission of Variola is by respiratory droplets, and MVA delivered by skin scarification (ss) protected mice far more effectively against lethal respiratory challenge with VACV than any other route of delivery, and at much lower doses. Comparisons of ss with intradermal, subcutaneous or intramuscular routes showed that MVAOVA ss-generated T cells were both more abundant and transcriptionally distinct. MVAOVA ss produced greater numbers of lung Ova-specific CD8+ TRM and was superior in protecting mice against lethal VACVOVA respiratory challenge. Nearly as many lung TRM were generated with MVAOVA ss compared to direct pulmonary immunization with MVAOVA, and both routes vaccination protected mice against lethal pulmonary challenge with VACVOVA. Strikingly, MVAOVA ss-generated effector T cells exhibited overlapping gene transcriptional profiles to those generated via direct pulmonary immunization. Overall, our data suggest that heterologous MVA vectors delivered via ss are uniquely well-suited as vaccine vectors for respiratory pathogens like COVID-19. In addition, MVA delivered via ss could represent a more effective dose-sparing smallpox vaccine.
2020-05-10 | GSE150190 | GEO
Project description:To study the effects of scarification on gene expression in the skin we performed microarray analysis on intact skin (control) and skin collected 2 h after scarification. Comparison of microarray data between the two groups showed that 451 probe sets (1.0% of a total 45038 probe sets on the arrays) representing 338 known genes were upregulated ≥ 2.0 fold in scarified skin samples when compared to controls. Further analyses of these genes showed that many were associated with an inflammatory immune response. Others were associated with wound-repair, response to stress, apoptosis and transcriptional regulation. The induction of high levels of genes encoding the neutrophil chemoattractants Cxcl1 and Cxcl2, the G-CSF receptor Csf3r, the GM-CSF receptor Csf2rb, the calcium binding proteins S100a8 and a9 (S100a8 and S100a9, respectively) the cytokines Ilb and Il6, and Cd14, Mmp9 and Sell, amongst many others, suggested a significant influx of neutrophillic granulocytes into the skin after scarification. Indeed, the expression of Cxcl2 and Il1b were up-regulated 50 fold in scarified skin when compared to intact skin. C57BL/6 mice (6-12 weeks old), 2-3 per group used. Prior to scarification approximately 1 cm2 area of hair covering the site of scarification was trimmed using curved scissors and then removed completely with an electric razor. Twenty-four hours later a 23-gauge needle was used to create a 5 mm long abrasion in the epidermal layers of the skin at the scarification site. 2 h later, ear skin was collected into RNAlater (Ambion, location) and stored at -80 oC prior to analysis. The quality of RNA for microarray analysis was first determined on an Agilent BioAnalyzer 2100 (Agilent Technologies). RNA was reverse transcribed to cDNA, amplified and labelled using the Genechip 3’ IVT Express Labelling Kit (Affymetrix, Santa Clara, CA, USA) according to the manufacturer’s instructions. Samples were hybridized to HT_MG-430_PM Affymetrix chips (Affymetrix), stained and scanned by ARK Genomics (ARK Genomics, Edinburgh, UK).
2012-03-01 | E-GEOD-34955 | biostudies-arrayexpress
Project description:To study the effects of scarification on gene expression in the skin we performed microarray analysis on intact skin (control) and skin collected 2 h after scarification. Comparison of microarray data between the two groups showed that 451 probe sets (1.0% of a total 45038 probe sets on the arrays) representing 338 known genes were upregulated ≥ 2.0 fold in scarified skin samples when compared to controls. Further analyses of these genes showed that many were associated with an inflammatory immune response. Others were associated with wound-repair, response to stress, apoptosis and transcriptional regulation. The induction of high levels of genes encoding the neutrophil chemoattractants Cxcl1 and Cxcl2, the G-CSF receptor Csf3r, the GM-CSF receptor Csf2rb, the calcium binding proteins S100a8 and a9 (S100a8 and S100a9, respectively) the cytokines Ilb and Il6, and Cd14, Mmp9 and Sell, amongst many others, suggested a significant influx of neutrophillic granulocytes into the skin after scarification. Indeed, the expression of Cxcl2 and Il1b were up-regulated 50 fold in scarified skin when compared to intact skin.
2012-03-01 | GSE34955 | GEO
Project description:Dermal macrophages (Macs) are essential for the timely resolution of staphylococcal skin infections. However the cell intrinsic pathways and microenvironmental cues that mediate this are unclear. Here we analyzed a strictly intradermal ear infection model with Staphylococcus aureus (S. aureus) to explore immunometabolic regulation.
2024-05-01 | GSE174675 | GEO
Project description:Dermal macrophages (Macs) are essential for the timely resolution of staphylococcal skin infections. However the cell intrinsic pathways and microenvironmental cues that mediate this are unclear. Here we analyzed a strictly intradermal ear infection model with Staphylococcus aureus (S. aureus) to explore immunometabolic regulation.
2024-05-01 | GSE174674 | GEO