Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression Profiling of Retinal Detachment and Accelerated Re-attachment

ABSTRACT: Retinal detachment is a major cause of blindness due to penetrating trauma and ocular inflammation, and is often observed in many patients following cataract extraction surgery. When the retinal photoreceptors detach from their epithelium, stress signals and apoptotic pathways are initiated that will lead to loss of vision, however accelerating the reattachment of these cells can prevent photoreceptor death and subsequent vision loss. To determine the genes involved in this process, we performed a microarray screen using a mouse model or retinal detachment in conjunction with a P2Y2 agonist previously demonstrated to hasten retinal reattachment. Keywords: disease state analysis and therapeutic analysis

ORGANISM(S): Mus musculus

PROVIDER: GSE5766 | GEO | 2007/11/10

SECONDARY ACCESSION(S): PRJNA97105

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of mouse with retinal detachment and accelerated re-attachment

Project description:Retinal detachment is a major cause of blindness due to penetrating trauma and ocular inflammation, and is often observed in many patients following cataract extraction surgery. When the retinal photoreceptors detach from their epithelium, stress signals and apoptotic pathways are initiated that will lead to loss of vision, however accelerating the reattachment of these cells can prevent photoreceptor death and subsequent vision loss. To determine the genes involved in this process, we performed a microarray screen using a mouse model or retinal detachment in conjunction with a P2Y2 agonist previously demonstrated to hasten retinal reattachment. Experiment Overall Design: We conducted a microarray screen to identify genes involved in promoting faster resolution of retinal detachment. Subretinal detachment was induced in Balb/cJ mice by subretinal injection of 1 uL saline or delivery of 1 uL of 10uM INS37217/Saline to cause detachment and expedite the rate of recovery. We performed this study at three timepoints: 2 hrs post-injection to identify early response genes; 24 hrs post-detachment when the retina has reattached, but still grossly misfolded; and 7 days post-detachment when the misfolding has been resolved, but retinal function is merely 50% of wild-type function. We used each RNA pool (each containing >5 retinas) for GeneChip hybridization giving a total of 3 biological replicates for each treatment at each timepoint. For each GeneChip, we labeled 7 ug of total RNA according to the manufacturerâs specifications (Affymetrix Inc.).

2008-06-14 | E-GEOD-5766 | biostudies-arrayexpress

Dual-function thermogelling polymers as effective endotamponade for retinal detachment repair and reassembly of vitreous-like body in-situ

Project description:Endo-tamponade agents are crucial surgical adjuncts for vitreo-retinal surgery. Current clinical agents provide internal tamponade through buoyancy forces requiring the need for prolonged facing down post-operative positioning. Other disadvantages include temporary loss of vision, raised intra-ocular pressure, cataract formation and a need for an additional removal surgery. Therein lies an unmet clinical need to develop alternative biomaterials to overcome these key challenges. We describe a biocompatible and biodegradable thermogelling polymer which provides an internal tamponade effect through surface tension and viscosity. Furthermore, we demonstrate its surgical efficacy in the repair of retinal detachment in a non-human primate model. By three-month post-surgery, the thermogel undergoes biodegradation, and is replaced by a vitreous-like body, thereby mimicking the biophysical needs of the natural vitreous body. In summary, we demonstrate the potential superiority of our thermogel compared to current vitreous tamponade gold standards; and present a significant advancement in the use of biomaterials for vitreo-retinal surgery.

2019-09-13 | PXD009525 | Pride

Transcriptomic analysis of human retinal detachment (RD) reveals both inflammation and photoreceptor death

Project description:We have used surgical specimens to perform a differential analysis of the transcriptome of human retinal tissues following detachment. Data analysis reveals major involvement of the immune response in the disease and interindividual variation was monitored to unravel a second crucial aspect of the pathological process, the death of photoreceptor cells. In total 38 surgical specimens (samples) were analyzed, 19 represent biopsies from patients with retinal detachment (RD) and 19 represent contral samples without retinal detachment.

2012-03-06 | E-GEOD-28133 | biostudies-arrayexpress

Gene differential expression between attachment and detachment of zebra mussel

Project description:A zebra mussel byssus cDNA microarray was used to identify the differentially expressed genes between attachment and detachment. Keywords: Gene differential expression

2008-04-19 | GSE10851 | GEO

Transcriptomic analysis of transferrin protective effect during retinal detachment in mice

Project description:Transcriptomic analysis of transferrin protective effect during retinal detachment in mice.

2019-08-31 | GSE102824 | GEO

Comparative Proteomic Analysis of retro-oil fluid and vitreous fluid from patients

Project description:There have been reports of unexplained visual loss following intra-ocular silicone oil (SiO) tamponade in retinal detachment patients, yet the underlying mechanism is unknown. In the present study, we identified differentially expressed proteins in the vitreous humor of four groups (each group, n=3): pars plana vitrectomy (PPV) for rhegmatogenous retinal detachment (RD group), SiO removal after successful retinal reattachment (SO group), cataract surgery after successful retinal reattachment with sterilized air tamponade (FA group), and PPV for epiretinal membrane (ERM group). DIA was combined with two-dimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS) to find expression changes in the proteome of vitreous.

2022-02-17 | PXD026816 | Pride

Gene differential expression between attachment and detachment of zebra mussel

Project description:A zebra mussel byssus cDNA microarray was used to identify the differentially expressed genes between attachment and detachment. Keywords: Gene differential expression The zebra mussels were divided in to two groups: AT and DT. In group AT all the zebra mussels were kept in the water attached for 48 hours while in group DT, the individuals were kept detached underwater for 48 hours. The feet of the zebra mussels were taken from each group. Four replicates, including four individual feet each replicate, were taken from each group for total RNA extraction. Four microarray hybridization performed between AT and DE. In each group, two cDNA replicates were labeled with Alexa 555 while the other two were labeled with Alexa 647. Every hybridization happened between two cDNA samples from different group with different labels.

2008-04-19 | E-GEOD-10851 | biostudies-arrayexpress

Efficacy of steroids in the treatment of retinal detachment with choroidal detachment: Analysis by proteomics

Project description:This study aims to utilize DIA-based proteomics technology to observe the effects of preoperative steroid interventions on the vitreous proteome in retinal detachment with choroidal detachment （RRDCD） patients. Gaining a deeper understanding of the changes in vitreous humor proteins following steroid-assisted therapy could offer valuable insights. Associating the proteomic profile of RRDCD with its clinical characteristics may provide new perspectives on the underlying mechanisms of the disease.

2024-12-03 | PXD058498 |

Single-Cell Multiomics Profiling Reveals Heterogeneity of Müller Cells in the Oxygen-Induced Retinopathy Model

Project description:Retinal neovascularization poses heightened risks of vision loss and blindness. Despite its clinical significance, the molecular mechanisms underlying the pathogenesis of retinal neovascularization remain elusive. This study utilized single-cell multiomics profiling in an oxygen-induced retinopathy (OIR) model to comprehensively investigate the intricate molecular landscape of retinal neovascularization.

2024-12-02 | GSE283276 | GEO

Molecular pathogenesis of rhegmatogenous retinal detachment

Project description:Here, we performed molecular pathology analysis of the vitreous proteomes collected from 127 patients with RRD using SWATH-mass spectrometry. For comparison, samples of neurodegenerative vitreoretinal interface eyes (MH, Pucker) and proliferative diabetic retinopathy eyes with tractional-retinal detachment (PDR-TRD) were used as a control.

2020-11-05 | MSV000086412 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data