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Deep Transcriptome Sequencing of a Rice Line Immune to Bacterial Blight Provides Novel Insights into Incompatible Interactions between Rice and Xanthomonas oryzae pv. oryzae

ABSTRACT: In this study, simultaneous gene expression, of both the host and pathogen, in the incompatible interaction between rice immune line H471 and Xoo race PXO99A with strong virulence, and in compatible ones between H471’s parents and PXO99A, were analyzed in the same infected leaf blades at 1 day and 3 days post-inoculation (dpi) using the RNA-Seq technique.This study provides the first insights into the differantial gene expression profiles of both organisms interacting simultaneously in the incompatible and compatible interactions between Xanthomonas oryzae pv. oryzae and host. Gene groups of differentially expressed genes were identified for PXO99^A and H471. In the Xoo case, a set of genes associated with two-component systems, involved in Quorum sensing (QS) and protecting the cell from reactive oxygen species (ROS) and oxidative stress, and genes encoding type III secretion systems and Type III (T3) effectors were sequentially up-regulated in the incompatible interaction. In the rice case, differentially regulated genes encoding a set of signal transduction components, sixty-six transcription factors, six genes encoding PPRs and three genes encoding tetratricopeptide repeat (TPR)-like superfamily proteins, and twenty-one resistance proteins have been identified in the incompatible interaction. Among them, six rice genes encoding response regulator receivers and histone proteins in the incompatible interaction uniquely differentially up-regulated in H471 at 1 dpi

ORGANISM(S): Oryza sativa Xanthomonas oryzae pv. oryzae

PROVIDER: GSE57670 | GEO | 2015/12/30

SECONDARY ACCESSION(S): PRJNA247488

REPOSITORIES: GEO

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Project description:X. oryzae pv. oryzae (Xoo) is the causal agent of bacterial blight of rice. X. oryzae pv. oryzicola (Xoc) is the causal agent of bacterial streak of rice. Fourteen day old rice leaves were inoculated with one of five strains of Xanthomonas oryzae. Seven strains of Xoo were used; three wild type strains (PXO99A, T7174, and PXO86) and strains PXO99AME7, which has a nonfunctional type III secretion system and is non-pathogenic, , PXO99AME1, a pthXo6 and avrXa27 double mutant, PXO99ME2, a pthXo1 mutant, and PXO99ME5, a reduced virulence strain with uncharacterized mutation in a TAL effector. One strain of Xoc (BLS303) was tested. Controls include an inoculation with water (MOCK) and no inoculation. T7174 is our label for the Japanese isolate MAFF311018. Third leaves are inoculated with a needless syringe at adjacent sites along the upper leaf blade. Six leaves from separate plants are pooled. RNA samples were collected 24 h after treatment. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Ginny Antony. The equivalent experiment is OS66 at PLEXdb.] cultivar: Nipponbare - strain: PXO99A(3-replications); cultivar: Nipponbare - strain: PXO99AME7(3-replications); cultivar: Nipponbare - strain: PXO99AME5(3-replications); cultivar: Nipponbare - strain: MOCK(3-replications); cultivar: Nipponbare - strain: NO TREATMENT(2-replications); cultivar: Nipponbare - strain: T7174(3-replications); cultivar: Nipponbare - strain: PXO86(3-replications); cultivar: Nipponbare - strain: BLS303(3-replications); cultivar: Nipponbare - strain: PXO99AME1(3-replications); cultivar: Nipponbare - strain: PXO99AME2(3-replications); cultivar: Nipponbare - strain: PXO99A-2(3-replications); cultivar: IR24 - strain: PXO99A(3-replications); cultivar: IR24 - strain: PXO99AME7(3-replications); cultivar: IR24 - strain: PXO99AME5(3-replications); cultivar: IR24 - strain: MOCK(3-replications); cultivar: IR24 - strain: NO TREATMENT(3-replications); cultivar: IR24 - strain: T7174(3-replications); cultivar: IR24 - strain: PXO86(3-replications); cultivar: IR24 - strain: BLS303(3-replications); cultivar: IR24 - strain: PXO99AME1(3-replications); cultivar: IR24 - strain: PXO99AME2(3-replications); cultivar: IR24 - strain: PXO99A-2(3-replications)

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Deep Transcriptome Sequencing of a Rice Line Immune to Bacterial Blight Provides Novel Insights into Incompatible Interactions between Rice and Xanthomonas oryzae pv. oryzae

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