Methylation profiling

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Genome-wide maps of DNA methylation state in rice embryo and endosperm


ABSTRACT: We report the application of methylacytosine immunoprecipetation combined with Illumina sequencing (MeDIP-seq) for high-throughput profiling of DNA methylation in rice embryo 3, 6, 9 DAP and endosperm 2, 3, 6, 9 DAP. A total number of 12-14 million of 2×49 bp paired-end reads was generated for each sample, and BOWTIE2 was used for read mapping. We generated genome-wide DNA methylation profiles of rice embryo and endosperm. This study provides a framework to systemically characterize the effect of DNA methylation in developing seeds and will help to illustrate the epigenetic regulation of rice seed development.

ORGANISM(S): Oryza sativa

PROVIDER: GSE57706 | GEO | 2015/09/01

SECONDARY ACCESSION(S): PRJNA247779

REPOSITORIES: GEO

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