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Transcriptomic effects of the expression in Arabidopsis of a TCP15-EAR fusion controlled by its own promoter (two-color experiment)

ABSTRACT: TCP proteins are transcription factors that participate in plant development (Martin-Trillo ad Cubas, 2010; Uberti-Manassero et al., 2013). Previous studies demonstrated that the Arabidopsis thaliana TCP protein TCP15 affects the development of leaves, stems and flowers (Kieffer et al., 2011; Uberti-Manassero et al., 2013). To obtain further insight into the molecular mechanisms involved in TCP15 action, we performed a transcriptional profiling of plants that express a repressor form of TCP15 under the control of the TCP15 promoter (P15::TCP15-EAR), previously described in Uberti-Manassero et al. (2012). For this purpose, gene expression was quantified in RNA extracted from complete rosettes of 25-day-old P15::TCP15-EAR plants and compared with that in RNA extracted from wild-type (Col-0) plants of the same age.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE57743 | GEO | 2015/05/16

SECONDARY ACCESSION(S): PRJNA247985

REPOSITORIES: GEO

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Transcriptomic effects of the expression in Arabidopsis of a TCP15-EAR fusion controlled by its own promoter (one-color experiment)

Project description:TCP proteins are transcription factors that participate in plant development (Martin-Trillo ad Cubas, 2010; Uberti-Manassero et al., 2013). Previous studies demonstrated that the Arabidopsis thaliana TCP protein TCP15 affects the development of leaves, stems and flowers (Kieffer et al., 2011; Uberti-Manassero et al., 2013). To obtain further insight into the molecular mechanisms involved in TCP15 action, we performed a transcriptional profiling of plants that express a repressor form of TCP15 under the control of the TCP15 promoter (P15::TCP15-EAR), previously described in Uberti-Manassero et al. (2012). For this purpose, gene expression was quantified in RNA extracted from complete rosettes of 25-day-old P15::TCP15-EAR plants and compared with that in RNA extracted from wild-type (Col-0) plants of the same age.

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