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3' RNA-seq of fly expressing cabut RNAi after exposure to different levels of sucrose

ABSTRACT: Control (+/cbtE1-UAS-cbt RNAi) or cabut RNAi flies (Tim-gal4, UAS-cbt RNAi) were starved for 16 hours and then exposed to food containing different concentrations of sucrose: 0, 25, 50 and 100 % for 18 hours. Fly heads were collected, RNA was extracted and RNA-seq libraries were prepared as previously described (Engreitz et al., 2013)

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE58854 | GEO | 2015/05/08

SECONDARY ACCESSION(S): PRJNA253720

REPOSITORIES: GEO

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3' RNA-seq of fly expressing cabut RNAi after exposure to different levels of sucrose

Project description:Control (+/cbtE1-UAS-cbt RNAi) or cabut RNAi flies (Tim-gal4, UAS-cbt RNAi) were starved for 16 hours and then exposed to food containing different concentrations of sucrose: 0, 25, 50 and 100 % for 18 hours. Fly heads were collected, RNA was extracted and RNA-seq libraries were prepared as previously described (Engreitz et al., 2013) For each sucrose concentration, two samples of cabut RNAi flies and one sample of control flies were sequenced.

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