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Thermal plasticity of the miRNA transcriptome during Senegalese sole development

ABSTRACT: We report the effect of incubation temperature on embryos and larvae of Solea senegalensis subjected to two different incubation temperatures (15 °C or 21 °C). We found that at some stages, a higher incubation temperature was associated with the expression of miRNAs positively related with growth

ORGANISM(S): Solea senegalensis

PROVIDER: GSE58297 | GEO | 2014/06/09

SECONDARY ACCESSION(S): PRJNA251943

REPOSITORIES: GEO

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Thermal plasticity of the miRNA transcriptome during Senegalese sole development

Project description:We report the effect of incubation temperature on embryos and larvae of Solea senegalensis subjected to two different incubation temperatures (15 Â°C or 21 Â°C). We found that at some stages, a higher incubation temperature was associated with the expression of miRNAs positively related with growth Examination of miRNA expression in Senegalese sole whole embryos and larvae subjected to two different incubation temperatures, using the SOLiD platform and validation by qPCR

2014-06-09 | E-GEOD-58297 | biostudies-arrayexpress

Solea senegalensis breed:senegalese sole

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Project description:Senegalese sole (Solea senegalensis) were treated with CuSO4 or lipopolysaccharide (LPS), an immune-system stimulant. Controls were treated with phosphate-buffered saline (PBS). Fish were sampled after 6 hours or 24 hours. Hepatic RNA from each treatment group was combined into pools, reverse transcribed to cDNA and labeled with Cy3-dCTP or Cy5-dCTP. Hybridisations consisted of a labeled pooled sample versus a reference mixture made from all PBS-treated samples. The microarray used was made from amplicons of European flounder (Platichthys flesus) hepatic cDNA.

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Thermal plasticity of the miRNA transcriptome during Senegalese sole development.

Project description: Not available

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Oogenesis in Solea senegalensis: various developmental stages

Project description:This SuperSeries is composed of the following subset Series: GSE17333: Oogenesis in Solea senegalensis: Vitellogenic versus mature ovaries GSE17334: Oogenesis in Solea senegalensis: vitellogenic versus atretic ovaries GSE17335: Oogenesis in Solea senegalensis: mature versus atretic ovaries GSE17336: Oogenesis in Solea senegalensis: Previtellogenic versus vitellogenic ovaries Refer to individual Series

2010-06-21 | E-GEOD-17337 | biostudies-arrayexpress

Molecular identification of genes involved in testicular steroid synthesis and characterization of the response to gonadotropic stimulation in the Senegalese sole (Solea senegalensis)

Project description:In male teleosts, testicular steroids are essential hormones for the regulation of spermatogenesis and their production is regulated by pituitary gonadotropins. In the Senegalese sole (Solea senegalensis), an economically important flatfish with semi-cystic and asynchronous spermatogenesis, the endocrine mechanisms involved in the regulation of spermatogenesis, particularly regarding the production and regulation of testicular steroids, are not well understood.This study aimed at describing the transcriptomic changes taking place in the Senegalese sole testis in response to hCG stimulation in vivo. Gene expression analysis by microarray identified 90 differentially expressed genes in the testis in response to hCG administration, including genes potentially involved in steroidogenesis, progression of spermatogenesis and germ cell maturation and cytoskeletal organization. Our results provide evidence for the first time that key genes involved in the regulation of steroid production and spermatogenesis in the Senegalese sole testis are under gonadotropic control.

2010-12-02 | GSE25733 | GEO

Transcriptional profiling of flatfish (Solea senegalensis) spermatogenesis

Project description:This SuperSeries is composed of the following subset Series: GSE21634: Transcriptional profiling of flatfish (Solea senegalensis) spermatogenesis_1 GSE21635: Transcriptional profiling of flatfish (Solea senegalensis) spermatogenesis_2 GSE21637: Transcriptional profiling of flatfish (Solea senegalensis) spermatogenesis_3 Refer to individual Series

2010-05-07 | E-GEOD-21694 | biostudies-arrayexpress

Molecular identification of genes involved in testicular steroid synthesis and characterization of the response to gonadotropic stimulation in the Senegalese sole (Solea senegalensis)

2010-12-02 | E-GEOD-25733 | biostudies-arrayexpress

Oogenesis in Solea senegalensis: vitellogenic versus atretic ovaries

Project description:Transcriptional profile changes in ovary during the reproductive cycle of Solea senegalensis: from vitellogenic stage to atresia stage obtained at different times during the annual reproductive cycle. An oligo microarray based on ESTs sequenced from S. senegalensis previously designed (Cerdà et al., 2008, BMC genomics) was used.

2009-10-02 | GSE17334 | GEO

Oogenesis in Solea senegalensis: Vitellogenic versus mature ovaries

Project description:Transcriptionnal profile changes in ovary during the reproductive cycle of Solea senegalensis: from vitellogenic stage to mature stage obtained at different times during the annual reproductive cycle. An oligo microarray based on ESTs sequenced from S. senegalensis previously designed (Cerdà et al., 2008, BMC genomics) was used.

2009-10-02 | GSE17333 | GEO

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