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RNA sequencing of mouse littermate wild-type and Shh null E10.5 forelimbs [Illumina]

ABSTRACT: We wanted to identify differentially expressed genes in wild-type and Shh null E10.5 mouse forelimbs

ORGANISM(S): Mus musculus

PROVIDER: GSE58645 | GEO | 2015/07/01

SECONDARY ACCESSION(S): PRJNA253094

REPOSITORIES: GEO

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RNA sequencing of mouse littermate wild-type and Shh null E10.5 forelimbs [Illumina]

Project description:We wanted to identify differentially expressed genes in wild-type and Shh null E10.5 mouse forelimbs Used two wild-type technical replicates and two Shh null technical replicates UT-Genome and Analysis Facility

2015-07-01 | E-GEOD-58645 | biostudies-arrayexpress

RNA sequencing of cultured mouse wild-type E10.25 forelimbs in control or cyclopmaine containing media [SOLiD]

2015-07-01 | GSE58664 | GEO

RNA sequencing of cultured mouse wild-type E10.25 forelimbs in control or cyclopmaine containing media [SOLiD]

Project description:We wanted to identify differentially expressed genes in wild-type forelimbs and forelimbs briefly exposed to Shh signaling. E10.25 forelimbs were cultured in control media and media containing cyclopmaine (inhibits the Hedgehog pathway) Generated two wild-type (control) and two cyclopmaine biological replicates for RNA-seq UT-Genome and Analysis Facility

2015-07-01 | E-GEOD-58664 | biostudies-arrayexpress

H3K27ac in WT and Shh-/-, and Shh-/- Gli3-/- E10.5 limb buds

Project description:H3K27ac in WT and Shh-/-, and Shh-/- Gli3-/- E10.5 limb buds

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Transcriptome of wild type and Shh limb buds at E10.5

Project description:The transcriptome of mouse limb buds of Shh mutant embryos was compared to the transcriptome of limb buds of wild type embryos at embryonic day E10.5

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ATAC-seq analysis to examine global accessibilty of chromatin in limb bud cells

Project description:The study compares two cell populations one where Etv2 is expressed and other lacks Etv2 expression. The cells from wild-type E9.5 hindlimbs , cells from Shh-EGFP E10.5 anterior hindlimbs , EGFP+ cells from Shh-EGFP E10.5 posterior hindlimbs, and EYFP+ cells from Etv2-EYFP transgenic E10.5 hindlimbs and forelimbs were collected and processed

2022-06-28 | GSE192865 | GEO

Chip-chip from Mouse E10.5 anterior and posterior immortalised limb cell lines and limb tissue for H3K27me3 antibody and from Mouse E10.5 anterior and posterior limb tissue for Ring1B antibody.

Project description:Anterior-posterior differences in H3K27me3 and Ring1B enrichment over the 5 prime Hoxd genes in E10.5 murine distal forelimbs. Chromatin immunoprecipitation (ChIP) of H3K27me3 together with Ring1B and by ChIP-on-chip analysis demonstrated that over the 5 prime HoxD locus H3K27me3 enrichment is decreased and Ring1B enrichment is sparse in limb cells derived from the distal posterior forelimb bud of E10.5 mouse embryos.

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The SBE7 enhancer is active in Shh expressing brain tissue at E10.5

Project description:The SBE7 enhancer is active in Shh expressing brain tissue at E10.5

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H3K27ac in WT and Shh-/-, and Shh-/-;Gli3-/- E10.5 limb buds

Project description:Purpose: The response to Hedgehog signaling in the limb is driven by GLI bound enhancers. While most Hh targets in the developing limb bud appear to be regulated by the activity of GLI-repressor, the role of GLI activator in this regulation is unclear. Here we assess the roles of GLI activator and repressor in regulating H3K27ac to activate their enhancers. Methods: ChIP-seq was performed in Embryonic day 10.5 mouse limb buds from WT, Shh-/- and Shh-/-Gli3-/- embryos Results: We found that most GLI binding regions can be activated in the absence of both GLI activator and repressor, while a small subset require GLI activator for activity. These indicate that most GLI enhancers in the limb are regulated by GLI repression.

2020-01-21 | GSE133712 | GEO

Expression data from E11.5 mouse embryonic forelimbs - various mutant conditions or dissected limb domains

Project description:Sonic hedgehog (Shh) signals via Gli transcription factors to direct digit number and identity in the vertebrate limb. We have characterized the Gli-dependent cis-regulatory network through a combination of whole genome ChIP-on-chip and transcriptional profiling of the developing mouse limb. In this dataset, we include the expression data obtained from dissected mouse forelimbs using a variety of gain- and loss-of-function hedgehog pathway mutants, as well as limbs dissected into responsive (posterior 2/3ds) and non-responsive (anterior 1/3d) Hh tissues. These data are used to obtain 753 genes that are differentially expressed in response to Shh signaling. Keywords: Comparison of genetic samples

2008-09-15 | GSE11063 | GEO

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